Mutation variants generated from nonvirulent coxsackievirus B3 acquire virulence phenotypes by active virus replication

Ilseon Seo, Youngmee Jee, Jeonghyun Ahn, Eun Jung Jun, Donghou Kim, Chul Hyun Joo, Yoo Kyum Kim, Heuiran Lee

Research output: Contribution to journalArticle

2 Scopus citations

Abstract

Objective: To understand coxsackievirus B3 (CVB3) virulence at the molecular level. Method: A mutation library was generated from noncardiovirulent CVB3/0. Highly virulent mutation variants were recovered and characterized both phenotypically and genotypically. Results: The variants consistently caused destruction of multiple tissues together with active virus production and induced severe mortality in vivo. The extent of infectious virus generation was directly correlated with that of histopathological aberration. Genotypic analysis of the entire genome indicated that the virulent viruses encode nucleotide substitutions in the 5′-nontranslated region, which have previously been identified in other virulent CVB3s. Conclusion: The present study provides evidence that particular nucleotide substitutions in the 5′-nontranslated region of nonvirulent CVB3 can lead to active virus replication, which is sufficient to induce virulence.

Original languageEnglish (US)
Pages (from-to)447-453
Number of pages7
JournalIntervirology
Volume50
Issue number6
DOIs
StatePublished - Feb 2008

Keywords

  • Coxsackievirus B3
  • Genotype analysis
  • Mutagenesis
  • Virus replication

ASJC Scopus subject areas

  • Virology
  • Infectious Diseases

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