Mutation variants generated from nonvirulent coxsackievirus B3 acquire virulence phenotypes by active virus replication

Ilseon Seo; Youngmee Jee; Jeonghyun Ahn; Eun Jung Jun; Donghou Kim; Chul Hyun Joo; Yoo Kyum Kim; Heuiran Lee

doi:10.1159/000115950

Mutation variants generated from nonvirulent coxsackievirus B3 acquire virulence phenotypes by active virus replication

Ilseon Seo, Youngmee Jee, Jeonghyun Ahn, Eun Jung Jun, Donghou Kim, Chul Hyun Joo, Yoo Kyum Kim, Heuiran Lee

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

Objective: To understand coxsackievirus B3 (CVB3) virulence at the molecular level. Method: A mutation library was generated from noncardiovirulent CVB3/0. Highly virulent mutation variants were recovered and characterized both phenotypically and genotypically. Results: The variants consistently caused destruction of multiple tissues together with active virus production and induced severe mortality in vivo. The extent of infectious virus generation was directly correlated with that of histopathological aberration. Genotypic analysis of the entire genome indicated that the virulent viruses encode nucleotide substitutions in the 5′-nontranslated region, which have previously been identified in other virulent CVB3s. Conclusion: The present study provides evidence that particular nucleotide substitutions in the 5′-nontranslated region of nonvirulent CVB3 can lead to active virus replication, which is sufficient to induce virulence.

Original language	English (US)
Pages (from-to)	447-453
Number of pages	7
Journal	Intervirology
Volume	50
Issue number	6
DOIs	https://doi.org/10.1159/000115950
State	Published - Feb 2008
Externally published	Yes

Keywords

Coxsackievirus B3
Genotype analysis
Mutagenesis
Virus replication

ASJC Scopus subject areas

Virology
Infectious Diseases

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title = "Mutation variants generated from nonvirulent coxsackievirus B3 acquire virulence phenotypes by active virus replication",

abstract = "Objective: To understand coxsackievirus B3 (CVB3) virulence at the molecular level. Method: A mutation library was generated from noncardiovirulent CVB3/0. Highly virulent mutation variants were recovered and characterized both phenotypically and genotypically. Results: The variants consistently caused destruction of multiple tissues together with active virus production and induced severe mortality in vivo. The extent of infectious virus generation was directly correlated with that of histopathological aberration. Genotypic analysis of the entire genome indicated that the virulent viruses encode nucleotide substitutions in the 5′-nontranslated region, which have previously been identified in other virulent CVB3s. Conclusion: The present study provides evidence that particular nucleotide substitutions in the 5′-nontranslated region of nonvirulent CVB3 can lead to active virus replication, which is sufficient to induce virulence.",

keywords = "Coxsackievirus B3, Genotype analysis, Mutagenesis, Virus replication",

author = "Ilseon Seo and Youngmee Jee and Jeonghyun Ahn and Jun, {Eun Jung} and Donghou Kim and Joo, {Chul Hyun} and Kim, {Yoo Kyum} and Heuiran Lee",

year = "2008",

month = feb,

doi = "10.1159/000115950",

language = "English (US)",

volume = "50",

pages = "447--453",

journal = "Intervirology",

issn = "0300-5526",

publisher = "S. Karger AG",

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TY - JOUR

T1 - Mutation variants generated from nonvirulent coxsackievirus B3 acquire virulence phenotypes by active virus replication

AU - Seo, Ilseon

AU - Jee, Youngmee

AU - Ahn, Jeonghyun

AU - Jun, Eun Jung

AU - Kim, Donghou

AU - Joo, Chul Hyun

AU - Kim, Yoo Kyum

AU - Lee, Heuiran

PY - 2008/2

Y1 - 2008/2

N2 - Objective: To understand coxsackievirus B3 (CVB3) virulence at the molecular level. Method: A mutation library was generated from noncardiovirulent CVB3/0. Highly virulent mutation variants were recovered and characterized both phenotypically and genotypically. Results: The variants consistently caused destruction of multiple tissues together with active virus production and induced severe mortality in vivo. The extent of infectious virus generation was directly correlated with that of histopathological aberration. Genotypic analysis of the entire genome indicated that the virulent viruses encode nucleotide substitutions in the 5′-nontranslated region, which have previously been identified in other virulent CVB3s. Conclusion: The present study provides evidence that particular nucleotide substitutions in the 5′-nontranslated region of nonvirulent CVB3 can lead to active virus replication, which is sufficient to induce virulence.

AB - Objective: To understand coxsackievirus B3 (CVB3) virulence at the molecular level. Method: A mutation library was generated from noncardiovirulent CVB3/0. Highly virulent mutation variants were recovered and characterized both phenotypically and genotypically. Results: The variants consistently caused destruction of multiple tissues together with active virus production and induced severe mortality in vivo. The extent of infectious virus generation was directly correlated with that of histopathological aberration. Genotypic analysis of the entire genome indicated that the virulent viruses encode nucleotide substitutions in the 5′-nontranslated region, which have previously been identified in other virulent CVB3s. Conclusion: The present study provides evidence that particular nucleotide substitutions in the 5′-nontranslated region of nonvirulent CVB3 can lead to active virus replication, which is sufficient to induce virulence.

KW - Coxsackievirus B3

KW - Genotype analysis

KW - Mutagenesis

KW - Virus replication

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