Abstract
Objective: To understand coxsackievirus B3 (CVB3) virulence at the molecular level. Method: A mutation library was generated from noncardiovirulent CVB3/0. Highly virulent mutation variants were recovered and characterized both phenotypically and genotypically. Results: The variants consistently caused destruction of multiple tissues together with active virus production and induced severe mortality in vivo. The extent of infectious virus generation was directly correlated with that of histopathological aberration. Genotypic analysis of the entire genome indicated that the virulent viruses encode nucleotide substitutions in the 5′-nontranslated region, which have previously been identified in other virulent CVB3s. Conclusion: The present study provides evidence that particular nucleotide substitutions in the 5′-nontranslated region of nonvirulent CVB3 can lead to active virus replication, which is sufficient to induce virulence.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 447-453 |
| Number of pages | 7 |
| Journal | Intervirology |
| Volume | 50 |
| Issue number | 6 |
| DOIs | |
| State | Published - Feb 1 2008 |
| Externally published | Yes |
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Keywords
- Coxsackievirus B3
- Genotype analysis
- Mutagenesis
- Virus replication
ASJC Scopus subject areas
- Virology
- Infectious Diseases
Cite this
Mutation variants generated from nonvirulent coxsackievirus B3 acquire virulence phenotypes by active virus replication. / Seo, Ilseon; Jee, Youngmee; Ahn, Jeonghyun; Jun, Eun Jung; Kim, Donghou; Joo, Chul Hyun; Kim, Yoo Kyum; Lee, Heuiran.
In: Intervirology, Vol. 50, No. 6, 01.02.2008, p. 447-453.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Mutation variants generated from nonvirulent coxsackievirus B3 acquire virulence phenotypes by active virus replication
AU - Seo, Ilseon
AU - Jee, Youngmee
AU - Ahn, Jeonghyun
AU - Jun, Eun Jung
AU - Kim, Donghou
AU - Joo, Chul Hyun
AU - Kim, Yoo Kyum
AU - Lee, Heuiran
PY - 2008/2/1
Y1 - 2008/2/1
N2 - Objective: To understand coxsackievirus B3 (CVB3) virulence at the molecular level. Method: A mutation library was generated from noncardiovirulent CVB3/0. Highly virulent mutation variants were recovered and characterized both phenotypically and genotypically. Results: The variants consistently caused destruction of multiple tissues together with active virus production and induced severe mortality in vivo. The extent of infectious virus generation was directly correlated with that of histopathological aberration. Genotypic analysis of the entire genome indicated that the virulent viruses encode nucleotide substitutions in the 5′-nontranslated region, which have previously been identified in other virulent CVB3s. Conclusion: The present study provides evidence that particular nucleotide substitutions in the 5′-nontranslated region of nonvirulent CVB3 can lead to active virus replication, which is sufficient to induce virulence.
AB - Objective: To understand coxsackievirus B3 (CVB3) virulence at the molecular level. Method: A mutation library was generated from noncardiovirulent CVB3/0. Highly virulent mutation variants were recovered and characterized both phenotypically and genotypically. Results: The variants consistently caused destruction of multiple tissues together with active virus production and induced severe mortality in vivo. The extent of infectious virus generation was directly correlated with that of histopathological aberration. Genotypic analysis of the entire genome indicated that the virulent viruses encode nucleotide substitutions in the 5′-nontranslated region, which have previously been identified in other virulent CVB3s. Conclusion: The present study provides evidence that particular nucleotide substitutions in the 5′-nontranslated region of nonvirulent CVB3 can lead to active virus replication, which is sufficient to induce virulence.
KW - Coxsackievirus B3
KW - Genotype analysis
KW - Mutagenesis
KW - Virus replication
UR - http://www.scopus.com/inward/record.url?scp=39749196721&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=39749196721&partnerID=8YFLogxK
U2 - 10.1159/000115950
DO - 10.1159/000115950
M3 - Article
C2 - 18268408
AN - SCOPUS:39749196721
VL - 50
SP - 447
EP - 453
JO - Intervirology
JF - Intervirology
SN - 0300-5526
IS - 6
ER -