Mutation of the Lck-binding motif of tip enhances lymphoid cell activation by herpesvirus saimiri

S. Monroe Duboise, Heuiran Lee, Jie Guo, Joong Kook Choi, Susan Czajak, Meredith Simon, Ronald Charles Desrosiers, Jae U. Jung

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

The proline-rich SH3-binding (SH3B) motif of the tyrosine kinase- interacting protein (Tip) of herpesvirus saimiri (HVS) is required for binding to the cellular Src family kinase Lck. We constructed a mutant form of HVS in which prolines in the SH3B motif of Tip were altered to alanines. This mutant form of Tip was incapable of binding to Lck. The mutant virus, HVS/Tip mSH3B, retained its ability to immortalize common marmoset lymphocytes in culture. In fact, common marmoset lymphocytes immortalized by the HVS/Tip mSH3B mutant displayed increased expression of HLA-DR lymphocyte activation marker, an altered pattern of tyrosine phosphorylation, increased expression of the tyrosine kinase Lyn, and a shift in electrophoretic mobility of Lck compared to cells immortalized by wild-type HVS. Experimental infection of common marmosets resulted in fulminant lymphoma with both HVS/Tip mSH3B and wild-type HVS. However, HVS/Tip mSH3B produced greater infiltration of affected organs by proliferating lymphoid cells compared to wild-type HVS. These results demonstrate that Tip binding to Lck is not necessary for transformation and that abrogation of Tip binding to Lck alters the characteristics of transformed cells and the severity of the pathologic lesions.

Original languageEnglish (US)
Pages (from-to)2607-2614
Number of pages8
JournalJournal of Virology
Volume72
Issue number4
StatePublished - Apr 1998
Externally publishedYes

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Saimiriine herpesvirus 2
Saimiriine Herpesvirus 2
Protein-Tyrosine Kinases
tyrosine
phosphotransferases (kinases)
Lymphocytes
mutation
Mutation
Callithrix
cells
Callithrix jacchus
proteins
mutants
protein binding
Proline
Protein Binding
proline
lymphocytes
src-Family Kinases
HLA-DR Antigens

ASJC Scopus subject areas

  • Immunology

Cite this

Duboise, S. M., Lee, H., Guo, J., Choi, J. K., Czajak, S., Simon, M., ... Jung, J. U. (1998). Mutation of the Lck-binding motif of tip enhances lymphoid cell activation by herpesvirus saimiri. Journal of Virology, 72(4), 2607-2614.

Mutation of the Lck-binding motif of tip enhances lymphoid cell activation by herpesvirus saimiri. / Duboise, S. Monroe; Lee, Heuiran; Guo, Jie; Choi, Joong Kook; Czajak, Susan; Simon, Meredith; Desrosiers, Ronald Charles; Jung, Jae U.

In: Journal of Virology, Vol. 72, No. 4, 04.1998, p. 2607-2614.

Research output: Contribution to journalArticle

Duboise, SM, Lee, H, Guo, J, Choi, JK, Czajak, S, Simon, M, Desrosiers, RC & Jung, JU 1998, 'Mutation of the Lck-binding motif of tip enhances lymphoid cell activation by herpesvirus saimiri', Journal of Virology, vol. 72, no. 4, pp. 2607-2614.
Duboise SM, Lee H, Guo J, Choi JK, Czajak S, Simon M et al. Mutation of the Lck-binding motif of tip enhances lymphoid cell activation by herpesvirus saimiri. Journal of Virology. 1998 Apr;72(4):2607-2614.
Duboise, S. Monroe ; Lee, Heuiran ; Guo, Jie ; Choi, Joong Kook ; Czajak, Susan ; Simon, Meredith ; Desrosiers, Ronald Charles ; Jung, Jae U. / Mutation of the Lck-binding motif of tip enhances lymphoid cell activation by herpesvirus saimiri. In: Journal of Virology. 1998 ; Vol. 72, No. 4. pp. 2607-2614.
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