Mutant p53 regulates ovarian cancer transformed phenotypes through autocrine matrix deposition

Marcin P. Iwanicki, Hsing Yu Chen, Claudia Iavarone, Ioannis K. Zervantonakis, Taru Muranen, Marián Novak, Tan Ince, Ronny Drapkin, Joan S. Brugge

Research output: Contribution to journalArticle

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Abstract

High-grade serous ovarian carcinoma (HGS-OvCa) harbors p53 mutations and can originate from the epithelial cell compartment of the fallopian tube fimbriae. From this site, neoplastic cells detach, survive in the peritoneal cavity, and form cellular clusters that intercalate into the mesothelium to form ovarian and peritoneal masses. To examine the contribution of mutant p53 to phenotypic alterations associated with HGS-OvCA, we developed live-cell microscopy assays that recapitulate these early events in cultured fallopian tube nonciliated epithelial (FNE) cells. Expression of stabilizing mutant variants of p53, but not depletion of endogenous wild-type p53, in FNE cells promoted survival and cell-cell aggregation under conditions of cell detachment, leading to the formation of cell clusters with mesothelium-intercalation capacity. Mutant p53R175H-induced phenotypes were dependent on fibronectin production, α5β1 fibronectin receptor engagement, and TWIST1 expression. These results indicate that FNE cells expressing stabilizing p53 mutants acquire anchorage independence and subsequent mesothelial intercalation capacity through a mechanism involving mesenchymal transition and matrix production. These findings provide important new insights into activities of mutant p53 in the cells of origin of HGS-OvCa.

Original languageEnglish (US)
Article numbere86829
JournalJCI Insight
Volume1
Issue number10
DOIs
StatePublished - Jul 7 2019

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Ovarian Neoplasms
Fallopian Tubes
Phenotype
Epithelial Cells
Epithelium
Integrin alpha5beta1
Carcinoma
Cell Aggregation
Peritoneal Cavity
Fibronectins
Microscopy
Cell Survival
Mutation

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Iwanicki, M. P., Chen, H. Y., Iavarone, C., Zervantonakis, I. K., Muranen, T., Novak, M., ... Brugge, J. S. (2019). Mutant p53 regulates ovarian cancer transformed phenotypes through autocrine matrix deposition. JCI Insight, 1(10), [e86829]. https://doi.org/10.1172/jci.insight.86829

Mutant p53 regulates ovarian cancer transformed phenotypes through autocrine matrix deposition. / Iwanicki, Marcin P.; Chen, Hsing Yu; Iavarone, Claudia; Zervantonakis, Ioannis K.; Muranen, Taru; Novak, Marián; Ince, Tan; Drapkin, Ronny; Brugge, Joan S.

In: JCI Insight, Vol. 1, No. 10, e86829, 07.07.2019.

Research output: Contribution to journalArticle

Iwanicki, MP, Chen, HY, Iavarone, C, Zervantonakis, IK, Muranen, T, Novak, M, Ince, T, Drapkin, R & Brugge, JS 2019, 'Mutant p53 regulates ovarian cancer transformed phenotypes through autocrine matrix deposition', JCI Insight, vol. 1, no. 10, e86829. https://doi.org/10.1172/jci.insight.86829
Iwanicki MP, Chen HY, Iavarone C, Zervantonakis IK, Muranen T, Novak M et al. Mutant p53 regulates ovarian cancer transformed phenotypes through autocrine matrix deposition. JCI Insight. 2019 Jul 7;1(10). e86829. https://doi.org/10.1172/jci.insight.86829
Iwanicki, Marcin P. ; Chen, Hsing Yu ; Iavarone, Claudia ; Zervantonakis, Ioannis K. ; Muranen, Taru ; Novak, Marián ; Ince, Tan ; Drapkin, Ronny ; Brugge, Joan S. / Mutant p53 regulates ovarian cancer transformed phenotypes through autocrine matrix deposition. In: JCI Insight. 2019 ; Vol. 1, No. 10.
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