Mutagenic Potential of a 193-nm Excimer Laser on Fibroblasts in Tissue Culture

Joseph Trentacoste, Keith Thompson, Richard K. Parrish, Anthony Hajek, Michael R. Berman, Parvin Ganjei

Research output: Contribution to journalArticle

28 Scopus citations

Abstract

In response to concerns regarding possible DNA damage by far ultraviolet radiation during excimer laser corneal surgery the mutagenic potential of an argon fluoride excimer laser (193 nm) on BALB/3T3 mouse fibroblasts grown in tissue culture was investigated. The cumulative incidence of anaplastic transformation after subablative radiant exposures from 3.5 mJ/cm2/pulse to 13.4 mJ/cm2/pulse was 3.6% of all cell cultures. The incidence of anaplastic transformation in nonirradiated controls was 4.2%. Transformation after exposure to x-ray radiation (60.9 rad) was 98.8%. The difference between the incidence of transformation of nonirradiated controls or excimer-treated cultures compared with x-ray radiation-treated cells was significant P < 0.0001 (chi square test). In this standard cell line, 193-nm laser energy does not appear to have substantial mutagenic potential.

Original languageEnglish (US)
Pages (from-to)125-129
Number of pages5
JournalOphthalmology
Volume94
Issue number2
DOIs
StatePublished - Jan 1 1987

Keywords

  • excimer laser
  • mutagenesis
  • photoablation

ASJC Scopus subject areas

  • Ophthalmology

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