Muscle ammonia stores are not determined by pH gradients

Patricia A. Wright, Chris M. Wood

Research output: Contribution to journalArticlepeer-review

23 Scopus citations


The theory of non-ionic diffusion predicts that ammonia will distribute between intracellular and extracellular tissue compartments according to transmembrane pH gradients. The distribution of ammonia and14C-DMO were compared in white muscle and plasma of rainbow trout (Salmo gairdneri) at rest, and following exhaustive exercise. Under both experimental conditions, intracellular ammonia levels far exceeded those predicted by transmembrane pH gradients. Calculated equilibrium potentials for {Mathematical expression}) were very close to published resting values of membrane potential Em in fish white muscle. We conclude that NH4+ is permeable across cell membranes and that intracellular ammonia stores are not determined by pH gradients.

Original languageEnglish (US)
Pages (from-to)159-162
Number of pages4
JournalFish Physiology and Biochemistry
Issue number3
StatePublished - Jul 1 1988


  • ammonia
  • fish
  • intracellular pH
  • white muscle

ASJC Scopus subject areas

  • Physiology (medical)
  • Physiology
  • Biochemistry
  • Biochemistry, Genetics and Molecular Biology(all)
  • Aquatic Science


Dive into the research topics of 'Muscle ammonia stores are not determined by pH gradients'. Together they form a unique fingerprint.

Cite this