TY - JOUR
T1 - Murine recombinant interleukin-3 induces recovery of T and B cells in irradiated mice
AU - Frasca, Daniela
AU - Leter, Giorgio
AU - Doria, Gino
PY - 1994/3/15
Y1 - 1994/3/15
N2 - Injection of murine recombinant interleukin-3 (IL-3) into (C57BL/10 x DBA/2) F1 mice, sublethally irradiated with 300 cGy and killed 14 days later, induced in the thymus recovery of the cell number and mitotic responsiveness to concanavalin A (Con A), as well as an increase in number of double- negative CD4-CD8-, double-positive CD4+CD8+, and single-positive CD4+CD8- and CD4-CD8+ cells. Also in the spleen, the cell count and mitotic responsiveness to Con A and lipopolysaccharide were increased to normal levels by IL-3 treatment. If the assays were performed 21 or 28 days after irradiation, IL-3 treatment was able to restore thymus and spleen cell counts as well as T- and B-cell mitotic responsiveness, even when mice were exposed to 400 or 500 cGy, respectively. These results altogether indicate that IL-3 induces differentiation and growth of thymocytes and recovery of T- and B-cell functions in mice exposed to sublethal irradiation.
AB - Injection of murine recombinant interleukin-3 (IL-3) into (C57BL/10 x DBA/2) F1 mice, sublethally irradiated with 300 cGy and killed 14 days later, induced in the thymus recovery of the cell number and mitotic responsiveness to concanavalin A (Con A), as well as an increase in number of double- negative CD4-CD8-, double-positive CD4+CD8+, and single-positive CD4+CD8- and CD4-CD8+ cells. Also in the spleen, the cell count and mitotic responsiveness to Con A and lipopolysaccharide were increased to normal levels by IL-3 treatment. If the assays were performed 21 or 28 days after irradiation, IL-3 treatment was able to restore thymus and spleen cell counts as well as T- and B-cell mitotic responsiveness, even when mice were exposed to 400 or 500 cGy, respectively. These results altogether indicate that IL-3 induces differentiation and growth of thymocytes and recovery of T- and B-cell functions in mice exposed to sublethal irradiation.
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U2 - 10.1182/blood.v83.6.1563.bloodjournal8361563
DO - 10.1182/blood.v83.6.1563.bloodjournal8361563
M3 - Article
C2 - 8123846
AN - SCOPUS:0028323184
VL - 83
SP - 1563
EP - 1568
JO - Blood
JF - Blood
SN - 0006-4971
IS - 6
ER -