Murine macrophage behavior on peptide-grafted polyethyleneglycol- containing networks

Weiyuan John Kao, Jeffrey A. Hubbell

Research output: Contribution to journalArticle

54 Citations (Scopus)

Abstract

Polyethyleneglycol-based networks were employed as substrates to graft bioactive peptides to study macrophage interactions with materials. Our overall objective was to utilize biologically active factors to stimulate certain macrophage function on materials suitable for implantation in connective tissues. In this study, we sought to explore the bioactivity of several peptides derived from extracellular matrix adhesion proteins and macrophage-active proteins that are normally soluble. The candidate peptides examined corresponded to residues 63 to 77 of complement component C3a (C3a((63-77))), residues 178 to 207 of interleukin-1 beta (IL1β((178- 207))), residues 1615 to 1624 of fibronectin (FN((1615-1624))), endothelial- macrophage activating polypeptide II, complement component C5a inhibitory sequence, macrophage inhibitory peptide, and YRGDG; materials lacking peptides were used as negative controls. An established murine cell-line IC- 21 was employed as a macrophage model, and human dermal fibroblasts were used for comparison. Our results showed that the substrates without grafted peptides were free from artifactual cell adhesion associated with the adsorption of serum or cellularly secreted proteins for long duration of culture. Of all grafted samples, IL1β((178-207))- and C3a((63-77))-grafted surfaces supported higher adherent macrophage densities. C3a((63-77))- and FN((1615-1624))-grafted surfaces supported higher adherent fibroblast densities. From competitive inhibition studies, cell adhesion was determined to occur in a receptor-peptide specific manner. The presence of grafted YRGDG in addition to IL1β((178-207)), C3a((63-77)), or FN((1615-1624)) synergistically increased macrophage and fibroblast adhesion. Materials grafted with IL1β((178-207)) or C3a((63-77)) co-grafted with or without YRGDG did not support the formation of multinucleated giant cells from the fusion of adherent macrophages in vitro.

Original languageEnglish
Pages (from-to)2-9
Number of pages8
JournalBiotechnology and Bioengineering
Volume59
Issue number1
DOIs
StatePublished - Jul 5 1998
Externally publishedYes

Fingerprint

Macrophages
Peptides
Fibroblasts
Cell adhesion
Proteins
Cell Adhesion
Complement C3a
Adhesion
Complement C5a
Peptide Receptors
Cell Fusion
Extracellular Matrix Proteins
Polypeptides
Substrates
Giant Cells
Bioactivity
Interleukin-1beta
Fibronectins
Grafts
Connective Tissue

Keywords

  • Cell adhesion
  • Fibroblasts
  • Multinucleated giant cell formation
  • Murine macrophages
  • Peptide immobilization
  • Polyethyleneglycol

ASJC Scopus subject areas

  • Biotechnology
  • Microbiology

Cite this

Murine macrophage behavior on peptide-grafted polyethyleneglycol- containing networks. / Kao, Weiyuan John; Hubbell, Jeffrey A.

In: Biotechnology and Bioengineering, Vol. 59, No. 1, 05.07.1998, p. 2-9.

Research output: Contribution to journalArticle

Kao, Weiyuan John ; Hubbell, Jeffrey A. / Murine macrophage behavior on peptide-grafted polyethyleneglycol- containing networks. In: Biotechnology and Bioengineering. 1998 ; Vol. 59, No. 1. pp. 2-9.
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