Abstract
A technique was developed to permit multiple large biopsies of the in vivo myocardium to measure certain metabolites (ATP, CP, myocardial glycogen, lactate) with minimal chance of ventricular fibrillation or severe alterations in the heart's ability to maintain BP. Adult pigs with normal hearts (n = 9) as well as with hypertrophy (n = 5) created by aortic banding 3-4 months prior were anesthetized, intubated, and ventilated. After a sternotomy, the pericardium was opened and the epicardial surface was cleared of any adhesions. Biopsies were taken from the distal to proximal end of the heart. A suture (3-0 Prolene) was placed in a figure-eight pattern in the area to be biopsied. A piece of the myocardium (50-100 mg) was grasped using a pair of narrow dressing forceps and frozen in liquid nitrogen. When the sample was completely frozen, a No. 11 scalpel blade, held at a 90° angle to the epicardium throughout the cut, was used to carve out the full thickness biopsy. The sample was placed immediately in liquid nitrogen. The myocardial defect was repaired by using the previously placed suture buttressed with pieces of subcutaneous fat to prevent tearing the epicardial surface. This in vivo biopsy method has negligible complications and can be repeated or taught with constant reliability.
| Original language | English |
|---|---|
| Pages (from-to) | 143-147 |
| Number of pages | 5 |
| Journal | Journal of Investigative Surgery |
| Volume | 5 |
| Issue number | 2 |
| DOIs | |
| State | Published - Jan 1 1992 |
| Externally published | Yes |
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Keywords
- Biopsies
- Freeze-clamping
- Myocardial
ASJC Scopus subject areas
- Surgery
Cite this
Multiple in vivo full-thickness myocardial biopsies by freeze-clamping. / Belanger, Michael P.; Torrance, Shona M.; Panos, Anthony; Wittnich, Carin.
In: Journal of Investigative Surgery, Vol. 5, No. 2, 01.01.1992, p. 143-147.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Multiple in vivo full-thickness myocardial biopsies by freeze-clamping
AU - Belanger, Michael P.
AU - Torrance, Shona M.
AU - Panos, Anthony
AU - Wittnich, Carin
PY - 1992/1/1
Y1 - 1992/1/1
N2 - A technique was developed to permit multiple large biopsies of the in vivo myocardium to measure certain metabolites (ATP, CP, myocardial glycogen, lactate) with minimal chance of ventricular fibrillation or severe alterations in the heart's ability to maintain BP. Adult pigs with normal hearts (n = 9) as well as with hypertrophy (n = 5) created by aortic banding 3-4 months prior were anesthetized, intubated, and ventilated. After a sternotomy, the pericardium was opened and the epicardial surface was cleared of any adhesions. Biopsies were taken from the distal to proximal end of the heart. A suture (3-0 Prolene) was placed in a figure-eight pattern in the area to be biopsied. A piece of the myocardium (50-100 mg) was grasped using a pair of narrow dressing forceps and frozen in liquid nitrogen. When the sample was completely frozen, a No. 11 scalpel blade, held at a 90° angle to the epicardium throughout the cut, was used to carve out the full thickness biopsy. The sample was placed immediately in liquid nitrogen. The myocardial defect was repaired by using the previously placed suture buttressed with pieces of subcutaneous fat to prevent tearing the epicardial surface. This in vivo biopsy method has negligible complications and can be repeated or taught with constant reliability.
AB - A technique was developed to permit multiple large biopsies of the in vivo myocardium to measure certain metabolites (ATP, CP, myocardial glycogen, lactate) with minimal chance of ventricular fibrillation or severe alterations in the heart's ability to maintain BP. Adult pigs with normal hearts (n = 9) as well as with hypertrophy (n = 5) created by aortic banding 3-4 months prior were anesthetized, intubated, and ventilated. After a sternotomy, the pericardium was opened and the epicardial surface was cleared of any adhesions. Biopsies were taken from the distal to proximal end of the heart. A suture (3-0 Prolene) was placed in a figure-eight pattern in the area to be biopsied. A piece of the myocardium (50-100 mg) was grasped using a pair of narrow dressing forceps and frozen in liquid nitrogen. When the sample was completely frozen, a No. 11 scalpel blade, held at a 90° angle to the epicardium throughout the cut, was used to carve out the full thickness biopsy. The sample was placed immediately in liquid nitrogen. The myocardial defect was repaired by using the previously placed suture buttressed with pieces of subcutaneous fat to prevent tearing the epicardial surface. This in vivo biopsy method has negligible complications and can be repeated or taught with constant reliability.
KW - Biopsies
KW - Freeze-clamping
KW - Myocardial
UR - http://www.scopus.com/inward/record.url?scp=0026508351&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0026508351&partnerID=8YFLogxK
U2 - 10.3109/08941939209012430
DO - 10.3109/08941939209012430
M3 - Article
C2 - 1610739
AN - SCOPUS:0026508351
VL - 5
SP - 143
EP - 147
JO - Journal of Investigative Surgery
JF - Journal of Investigative Surgery
SN - 0894-1939
IS - 2
ER -