Multiple exoribonucleases are required for the 3′ processing of Escherichia coli tRNA precursors in vivo

Nina Bacher Reuven, Murray P Deutscher

Research output: Contribution to journalArticle

54 Citations (Scopus)

Abstract

Our knowledge of the 3′ processing of tRNA precursors is severely limited. Although six exoribonucleases able to act on Escherichia coli tRNA precursors in vitro have been identified, their involvement in tRNA maturation in vivo has not been demonstrated. Here we show, using a wide range of multiple RNase-deficient strains and a quantitative suppression assay, that at least five of these enzymes -RNase II, RNase D, RNase BN, RNase T, and RNase PH-can participate in the synthesis of functional tRNATyrsu+3 in vivo. Moreover, any one of the five RNases is sufficient to allow tRNA processing to proceed although with varying effectiveness. Examination of the level of aminoacylation of tRNA isolated from RNase-deficient strains suggested that tRNA precursors accumulate in the most defective cells. These data indicate that exoribonucleases are required for tRNA maturation in vivo and that there is a high degree of functional overlap among the enzymes. These studies contribute to the identification of all the enzymes necessary for defining the complete processing pathway for E. coli tRNA precursors.

Original languageEnglish
Pages (from-to)143-148
Number of pages6
JournalFASEB Journal
Volume7
Issue number1
StatePublished - Jan 1 1993
Externally publishedYes

Fingerprint

exoribonucleases
Exoribonucleases
RNA Precursors
Transfer RNA
Escherichia coli
Ribonucleases
Processing
Enzymes
enzymes
aminoacylation
Transfer RNA Aminoacylation
Ribonuclease III
cans
Assays
synthesis
poly A specific exoribonuclease
RNA precursors
assays
cells

Keywords

  • Suppression
  • tRNA maturation

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Cell Biology

Cite this

Multiple exoribonucleases are required for the 3′ processing of Escherichia coli tRNA precursors in vivo. / Reuven, Nina Bacher; Deutscher, Murray P.

In: FASEB Journal, Vol. 7, No. 1, 01.01.1993, p. 143-148.

Research output: Contribution to journalArticle

Reuven, Nina Bacher ; Deutscher, Murray P. / Multiple exoribonucleases are required for the 3′ processing of Escherichia coli tRNA precursors in vivo. In: FASEB Journal. 1993 ; Vol. 7, No. 1. pp. 143-148.
@article{d3d1230ba2724a51b0b63698cb31cda2,
title = "Multiple exoribonucleases are required for the 3′ processing of Escherichia coli tRNA precursors in vivo",
abstract = "Our knowledge of the 3′ processing of tRNA precursors is severely limited. Although six exoribonucleases able to act on Escherichia coli tRNA precursors in vitro have been identified, their involvement in tRNA maturation in vivo has not been demonstrated. Here we show, using a wide range of multiple RNase-deficient strains and a quantitative suppression assay, that at least five of these enzymes -RNase II, RNase D, RNase BN, RNase T, and RNase PH-can participate in the synthesis of functional tRNATyrsu+3 in vivo. Moreover, any one of the five RNases is sufficient to allow tRNA processing to proceed although with varying effectiveness. Examination of the level of aminoacylation of tRNA isolated from RNase-deficient strains suggested that tRNA precursors accumulate in the most defective cells. These data indicate that exoribonucleases are required for tRNA maturation in vivo and that there is a high degree of functional overlap among the enzymes. These studies contribute to the identification of all the enzymes necessary for defining the complete processing pathway for E. coli tRNA precursors.",
keywords = "Suppression, tRNA maturation",
author = "Reuven, {Nina Bacher} and Deutscher, {Murray P}",
year = "1993",
month = "1",
day = "1",
language = "English",
volume = "7",
pages = "143--148",
journal = "FASEB Journal",
issn = "0892-6638",
publisher = "FASEB",
number = "1",

}

TY - JOUR

T1 - Multiple exoribonucleases are required for the 3′ processing of Escherichia coli tRNA precursors in vivo

AU - Reuven, Nina Bacher

AU - Deutscher, Murray P

PY - 1993/1/1

Y1 - 1993/1/1

N2 - Our knowledge of the 3′ processing of tRNA precursors is severely limited. Although six exoribonucleases able to act on Escherichia coli tRNA precursors in vitro have been identified, their involvement in tRNA maturation in vivo has not been demonstrated. Here we show, using a wide range of multiple RNase-deficient strains and a quantitative suppression assay, that at least five of these enzymes -RNase II, RNase D, RNase BN, RNase T, and RNase PH-can participate in the synthesis of functional tRNATyrsu+3 in vivo. Moreover, any one of the five RNases is sufficient to allow tRNA processing to proceed although with varying effectiveness. Examination of the level of aminoacylation of tRNA isolated from RNase-deficient strains suggested that tRNA precursors accumulate in the most defective cells. These data indicate that exoribonucleases are required for tRNA maturation in vivo and that there is a high degree of functional overlap among the enzymes. These studies contribute to the identification of all the enzymes necessary for defining the complete processing pathway for E. coli tRNA precursors.

AB - Our knowledge of the 3′ processing of tRNA precursors is severely limited. Although six exoribonucleases able to act on Escherichia coli tRNA precursors in vitro have been identified, their involvement in tRNA maturation in vivo has not been demonstrated. Here we show, using a wide range of multiple RNase-deficient strains and a quantitative suppression assay, that at least five of these enzymes -RNase II, RNase D, RNase BN, RNase T, and RNase PH-can participate in the synthesis of functional tRNATyrsu+3 in vivo. Moreover, any one of the five RNases is sufficient to allow tRNA processing to proceed although with varying effectiveness. Examination of the level of aminoacylation of tRNA isolated from RNase-deficient strains suggested that tRNA precursors accumulate in the most defective cells. These data indicate that exoribonucleases are required for tRNA maturation in vivo and that there is a high degree of functional overlap among the enzymes. These studies contribute to the identification of all the enzymes necessary for defining the complete processing pathway for E. coli tRNA precursors.

KW - Suppression

KW - tRNA maturation

UR - http://www.scopus.com/inward/record.url?scp=0027393081&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0027393081&partnerID=8YFLogxK

M3 - Article

C2 - 8422961

AN - SCOPUS:0027393081

VL - 7

SP - 143

EP - 148

JO - FASEB Journal

JF - FASEB Journal

SN - 0892-6638

IS - 1

ER -