Multimodal enzymatic sensing for continuous wound monitoring

S. RoyChoudhury; P. Bhushan; Y. Umasankar; P. A. Hirt; H. A. Lev-Tov; R. S. Kirsner; S. Bhansali

doi:10.1149/08801.0419ecst

Multimodal enzymatic sensing for continuous wound monitoring

S. RoyChoudhury, P. Bhushan, Y. Umasankar, P. A. Hirt, H. A. Lev-Tov, R. S. Kirsner, S. Bhansali

Dermatology & Cutaneous Surgery

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

Present wound care is primarily visual, entailing repeated clinical trips. A biosensing system offering direct longitudinal measures of healing can assist to bridge the time between occurrence of wounds and their management. Purine metabolites, xanthine and uric acid, are known to have high correlation with wound chronicity and healing. With increased apoptosis, concentrations of these analytes reportedly increase. In this study, their biochemical pathways in purine metabolism are explored to provide a method to monitor healing on a multimodal sensing platform. This work employs enzymes xanthine oxidase and uricase, for detection of uric acid and xanthine respectively. pH of wounds change with healing, from alkaline to acidic, shifting the potential of detection. Effects of pH have been investigated on the same platform enabling real-time calibration, attempting to provide improved assessment of tissue recovery. Nanomaterials of carbon (C) and gold (Au) have been utilized here, to improve the sensitivity of detection.

Original language	English (US)
Title of host publication	ECS Transactions
Editors	A. Khosla, H. Furukawa, K. Fukushima, K. Ito, A. Kakugo, S. Tokito, T.-Y. Chu, H. Matsui, F. D. Dos Santos, T. Shiba, H. Ito, A. Nakai, T. Saito, G. Milano, Y. Masubuchi, T. Yoshida, M. White, M. Yanagida, M. Kaltenbrunner, A. Masuhara, S. Bhansali, L. Nagahara, P. Sekhar
Publisher	Electrochemical Society Inc.
Pages	419-426
Number of pages	8
Edition	1
ISBN (Electronic)	9781607688655
DOIs	https://doi.org/10.1149/08801.0419ecst
State	Published - 2018
Event	1st International Conference on 4D Materials and Systems, 4DMS 2018 - Yonezawa, Japan Duration: Aug 26 2018 → Aug 30 2018

Publication series

Name	ECS Transactions
Number	1
Volume	88
ISSN (Print)	1938-6737
ISSN (Electronic)	1938-5862

Conference

Conference	1st International Conference on 4D Materials and Systems, 4DMS 2018
Country/Territory	Japan
City	Yonezawa
Period	8/26/18 → 8/30/18

ASJC Scopus subject areas

Engineering(all)

Access to Document

10.1149/08801.0419ecst

Cite this

RoyChoudhury, S., Bhushan, P., Umasankar, Y., Hirt, P. A., Lev-Tov, H. A., Kirsner, R. S., & Bhansali, S. (2018). Multimodal enzymatic sensing for continuous wound monitoring. In A. Khosla, H. Furukawa, K. Fukushima, K. Ito, A. Kakugo, S. Tokito, T-Y. Chu, H. Matsui, F. D. Dos Santos, T. Shiba, H. Ito, A. Nakai, T. Saito, G. Milano, Y. Masubuchi, T. Yoshida, M. White, M. Yanagida, M. Kaltenbrunner, A. Masuhara, S. Bhansali, L. Nagahara, ... P. Sekhar (Eds.), ECS Transactions (1 ed., pp. 419-426). (ECS Transactions; Vol. 88, No. 1). Electrochemical Society Inc.. https://doi.org/10.1149/08801.0419ecst

Multimodal enzymatic sensing for continuous wound monitoring. / RoyChoudhury, S.; Bhushan, P.; Umasankar, Y.; Hirt, P. A.; Lev-Tov, H. A.; Kirsner, R. S.; Bhansali, S.

ECS Transactions. ed. / A. Khosla; H. Furukawa; K. Fukushima; K. Ito; A. Kakugo; S. Tokito; T.-Y. Chu; H. Matsui; F. D. Dos Santos; T. Shiba; H. Ito; A. Nakai; T. Saito; G. Milano; Y. Masubuchi; T. Yoshida; M. White; M. Yanagida; M. Kaltenbrunner; A. Masuhara; S. Bhansali; L. Nagahara; P. Sekhar. 1. ed. Electrochemical Society Inc., 2018. p. 419-426 (ECS Transactions; Vol. 88, No. 1).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

RoyChoudhury, S, Bhushan, P, Umasankar, Y, Hirt, PA, Lev-Tov, HA , Kirsner, RS & Bhansali, S 2018, Multimodal enzymatic sensing for continuous wound monitoring. in A Khosla, H Furukawa, K Fukushima, K Ito, A Kakugo, S Tokito, T-Y Chu, H Matsui, FD Dos Santos, T Shiba, H Ito, A Nakai, T Saito, G Milano, Y Masubuchi, T Yoshida, M White, M Yanagida, M Kaltenbrunner, A Masuhara, S Bhansali, L Nagahara & P Sekhar (eds), ECS Transactions. 1 edn, ECS Transactions, no. 1, vol. 88, Electrochemical Society Inc., pp. 419-426, 1st International Conference on 4D Materials and Systems, 4DMS 2018, Yonezawa, Japan, 8/26/18. https://doi.org/10.1149/08801.0419ecst

RoyChoudhury S, Bhushan P, Umasankar Y, Hirt PA, Lev-Tov HA , Kirsner RS et al. Multimodal enzymatic sensing for continuous wound monitoring. In Khosla A, Furukawa H, Fukushima K, Ito K, Kakugo A, Tokito S, Chu T-Y, Matsui H, Dos Santos FD, Shiba T, Ito H, Nakai A, Saito T, Milano G, Masubuchi Y, Yoshida T, White M, Yanagida M, Kaltenbrunner M, Masuhara A, Bhansali S, Nagahara L, Sekhar P, editors, ECS Transactions. 1 ed. Electrochemical Society Inc. 2018. p. 419-426. (ECS Transactions; 1). https://doi.org/10.1149/08801.0419ecst

RoyChoudhury, S. ; Bhushan, P. ; Umasankar, Y. ; Hirt, P. A. ; Lev-Tov, H. A. ; Kirsner, R. S. ; Bhansali, S. / Multimodal enzymatic sensing for continuous wound monitoring. ECS Transactions. editor / A. Khosla ; H. Furukawa ; K. Fukushima ; K. Ito ; A. Kakugo ; S. Tokito ; T.-Y. Chu ; H. Matsui ; F. D. Dos Santos ; T. Shiba ; H. Ito ; A. Nakai ; T. Saito ; G. Milano ; Y. Masubuchi ; T. Yoshida ; M. White ; M. Yanagida ; M. Kaltenbrunner ; A. Masuhara ; S. Bhansali ; L. Nagahara ; P. Sekhar. 1. ed. Electrochemical Society Inc., 2018. pp. 419-426 (ECS Transactions; 1).

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abstract = "Present wound care is primarily visual, entailing repeated clinical trips. A biosensing system offering direct longitudinal measures of healing can assist to bridge the time between occurrence of wounds and their management. Purine metabolites, xanthine and uric acid, are known to have high correlation with wound chronicity and healing. With increased apoptosis, concentrations of these analytes reportedly increase. In this study, their biochemical pathways in purine metabolism are explored to provide a method to monitor healing on a multimodal sensing platform. This work employs enzymes xanthine oxidase and uricase, for detection of uric acid and xanthine respectively. pH of wounds change with healing, from alkaline to acidic, shifting the potential of detection. Effects of pH have been investigated on the same platform enabling real-time calibration, attempting to provide improved assessment of tissue recovery. Nanomaterials of carbon (C) and gold (Au) have been utilized here, to improve the sensitivity of detection.",

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Multimodal enzymatic sensing for continuous wound monitoring

Abstract

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ASJC Scopus subject areas

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