Multicolumn high resolution LC-MALDI MS system for high throughput analysis

T. Rejtar, A. R. Ivanov, H. S. Chen, V. Andreev, E. Moskovets, B. L. Karger

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

A multicolumn high resolution LC-MALDI MS system was developed for high throughput proteomic analysis. Complex samples were fractionated by strong cation exchange chromatography into 20 or more fractions. The UltiMate nano-LC system was optimized to operate in the automatic multicolumn nano-LC mode. The use of peptide trap precolumns allowed preconcentration of diluted samples and rapid loading of the sample at flow rate of up to 5 μl/min. The MS spectra were processed using cross-correlation algorithm and m/z values corresponding to peaks with chromatographic behavior were selected for peptide mass fingerprinting and/or MS/MS interrogation.

Original languageEnglish
Title of host publicationProceedings 50th ASMS Conference on Mass Spectrometry and Allied Topics
Pages193-194
Number of pages2
StatePublished - Dec 1 2002
EventProceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics - Orlando, FL, United States
Duration: Jun 2 2002Jun 6 2002

Other

OtherProceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics
CountryUnited States
CityOrlando, FL
Period6/2/026/6/02

Fingerprint

Throughput
Peptides
Chromatography
Cations
Flow rate
Proteomics

ASJC Scopus subject areas

  • Spectroscopy

Cite this

Rejtar, T., Ivanov, A. R., Chen, H. S., Andreev, V., Moskovets, E., & Karger, B. L. (2002). Multicolumn high resolution LC-MALDI MS system for high throughput analysis. In Proceedings 50th ASMS Conference on Mass Spectrometry and Allied Topics (pp. 193-194)

Multicolumn high resolution LC-MALDI MS system for high throughput analysis. / Rejtar, T.; Ivanov, A. R.; Chen, H. S.; Andreev, V.; Moskovets, E.; Karger, B. L.

Proceedings 50th ASMS Conference on Mass Spectrometry and Allied Topics. 2002. p. 193-194.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Rejtar, T, Ivanov, AR, Chen, HS, Andreev, V, Moskovets, E & Karger, BL 2002, Multicolumn high resolution LC-MALDI MS system for high throughput analysis. in Proceedings 50th ASMS Conference on Mass Spectrometry and Allied Topics. pp. 193-194, Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics, Orlando, FL, United States, 6/2/02.
Rejtar T, Ivanov AR, Chen HS, Andreev V, Moskovets E, Karger BL. Multicolumn high resolution LC-MALDI MS system for high throughput analysis. In Proceedings 50th ASMS Conference on Mass Spectrometry and Allied Topics. 2002. p. 193-194
Rejtar, T. ; Ivanov, A. R. ; Chen, H. S. ; Andreev, V. ; Moskovets, E. ; Karger, B. L. / Multicolumn high resolution LC-MALDI MS system for high throughput analysis. Proceedings 50th ASMS Conference on Mass Spectrometry and Allied Topics. 2002. pp. 193-194
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