Multicity outbreak of carbapenem-resistant Acinetobacter baumannii isolates producing the carbapenemase OXA-40

Karen Lolans, Thomas W. Rice, Luisa Munoz-Price, John P. Quinn

Research output: Contribution to journalArticle

152 Citations (Scopus)

Abstract

During 2005 we detected a multicity outbreak of infections or colonization due to high-level imipenem-resistant Acinetobacter baumannii (MIC, 64 μg/ml). One hundred isolates from diverse sources were obtained from seven acute-care hospitals and two extended-care facilities; 97% of the isolates belonged to one clone. Susceptibility testing of the first 42 isolates (January to April 2005) revealed broad resistance profiles. Half of the isolates were susceptible to ceftazidime, with many isolates susceptible only to colistin. The level of AmpC β-lactamase expression was stronger in isolates resistant to ceftazidime. PCR and subsequent nucleotide sequencing analysis identified bla OXA-40. The presence of an OXA-40 β-lactamase in these isolates correlated with the carbapenem resistance. By Southern blot analysis, a bla OXA-40-specific probe revealed that the gene was both plasmid and chromosomally located. This is the first time in the United States that such carbapenem resistance in A. baumannii has been attributable to a carbapenemase.

Original languageEnglish
Pages (from-to)2941-2945
Number of pages5
JournalAntimicrobial Agents and Chemotherapy
Volume50
Issue number9
DOIs
StatePublished - Sep 1 2006
Externally publishedYes

Fingerprint

Acinetobacter baumannii
Carbapenems
Disease Outbreaks
Ceftazidime
Skilled Nursing Facilities
Colistin
Imipenem
Southern Blotting
Plasmids
Nucleotides
Clone Cells
Polymerase Chain Reaction
Infection
Genes
Asp(5)-oxytocin
carbapenemase

ASJC Scopus subject areas

  • Pharmacology (medical)

Cite this

Multicity outbreak of carbapenem-resistant Acinetobacter baumannii isolates producing the carbapenemase OXA-40. / Lolans, Karen; Rice, Thomas W.; Munoz-Price, Luisa; Quinn, John P.

In: Antimicrobial Agents and Chemotherapy, Vol. 50, No. 9, 01.09.2006, p. 2941-2945.

Research output: Contribution to journalArticle

Lolans, Karen ; Rice, Thomas W. ; Munoz-Price, Luisa ; Quinn, John P. / Multicity outbreak of carbapenem-resistant Acinetobacter baumannii isolates producing the carbapenemase OXA-40. In: Antimicrobial Agents and Chemotherapy. 2006 ; Vol. 50, No. 9. pp. 2941-2945.
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