Multi-locus sequence typing provides epidemiological insights for diseased sharks infected with fungi belonging to the Fusarium solani species complex

Guillaume Desoubeaux, Anne Debourgogne, Nathan P. Wiederhold, Marie Zaffino, Deanna Sutton, Rachel E. Burns, Salvatore Frasca, Michael W. Hyatt, Carolyn Cray

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Fusarium spp. are saprobic moulds that are responsible for severe opportunistic infections in humans and animals. However, we need epidemiological tools to reliably trace the circulation of such fungal strains within medical or veterinary facilities, to recognize environmental contaminations that might lead to infection and to improve our understanding of factors responsible for the onset of outbreaks. In this study, we used molecular genotyping to investigate clustered cases of Fusarium solani species complex (FSSC) infection that occurred in eight Sphyrnidae sharks under managed care at a public aquarium. Genetic relationships between fungal strains were determined by multi-locus sequence typing (MLST) analysis based on DNA sequencing at five loci, followed by comparison with sequences of 50 epidemiologically unrelated FSSC strains. Our genotyping approach revealed that F. keratoplasticum and F. solani haplotype 9x were most commonly isolated. In one case, the infection proved to be with another Hypocrealian rare opportunistic pathogen Metarhizium robertsii. Twice, sharks proved to be infected with FSSC strains with the same MLST sequence type, supporting the hypothesis the hypothesis that common environmental populations of fungi existed for these sharks and would suggest the longtime persistence of the two clonal strains within the environment, perhaps in holding pools and life support systems of the aquarium. This study highlights how molecular tools like MLST can be used to investigate outbreaks of microbiological disease. This work reinforces the need for regular controls of water quality to reduce microbiological contamination due to waterborne microorganisms.

Original languageEnglish (US)
Pages (from-to)591-601
Number of pages11
JournalMedical Mycology
Volume56
Issue number5
DOIs
StatePublished - Jul 1 2018

Fingerprint

Sharks
Fusarium
Fungi
Disease Outbreaks
Life Support Systems
Infection
Metarhizium
Water Quality
Opportunistic Infections
Managed Care Programs
DNA Sequence Analysis
Haplotypes
Sequence Analysis
Population

ASJC Scopus subject areas

  • Infectious Diseases

Cite this

Multi-locus sequence typing provides epidemiological insights for diseased sharks infected with fungi belonging to the Fusarium solani species complex. / Desoubeaux, Guillaume; Debourgogne, Anne; Wiederhold, Nathan P.; Zaffino, Marie; Sutton, Deanna; Burns, Rachel E.; Frasca, Salvatore; Hyatt, Michael W.; Cray, Carolyn.

In: Medical Mycology, Vol. 56, No. 5, 01.07.2018, p. 591-601.

Research output: Contribution to journalArticle

Desoubeaux, G, Debourgogne, A, Wiederhold, NP, Zaffino, M, Sutton, D, Burns, RE, Frasca, S, Hyatt, MW & Cray, C 2018, 'Multi-locus sequence typing provides epidemiological insights for diseased sharks infected with fungi belonging to the Fusarium solani species complex', Medical Mycology, vol. 56, no. 5, pp. 591-601. https://doi.org/10.1093/mmy/myx089
Desoubeaux, Guillaume ; Debourgogne, Anne ; Wiederhold, Nathan P. ; Zaffino, Marie ; Sutton, Deanna ; Burns, Rachel E. ; Frasca, Salvatore ; Hyatt, Michael W. ; Cray, Carolyn. / Multi-locus sequence typing provides epidemiological insights for diseased sharks infected with fungi belonging to the Fusarium solani species complex. In: Medical Mycology. 2018 ; Vol. 56, No. 5. pp. 591-601.
@article{cee7f88f1ac345c6b15bb7cc0c153c25,
title = "Multi-locus sequence typing provides epidemiological insights for diseased sharks infected with fungi belonging to the Fusarium solani species complex",
abstract = "Fusarium spp. are saprobic moulds that are responsible for severe opportunistic infections in humans and animals. However, we need epidemiological tools to reliably trace the circulation of such fungal strains within medical or veterinary facilities, to recognize environmental contaminations that might lead to infection and to improve our understanding of factors responsible for the onset of outbreaks. In this study, we used molecular genotyping to investigate clustered cases of Fusarium solani species complex (FSSC) infection that occurred in eight Sphyrnidae sharks under managed care at a public aquarium. Genetic relationships between fungal strains were determined by multi-locus sequence typing (MLST) analysis based on DNA sequencing at five loci, followed by comparison with sequences of 50 epidemiologically unrelated FSSC strains. Our genotyping approach revealed that F. keratoplasticum and F. solani haplotype 9x were most commonly isolated. In one case, the infection proved to be with another Hypocrealian rare opportunistic pathogen Metarhizium robertsii. Twice, sharks proved to be infected with FSSC strains with the same MLST sequence type, supporting the hypothesis the hypothesis that common environmental populations of fungi existed for these sharks and would suggest the longtime persistence of the two clonal strains within the environment, perhaps in holding pools and life support systems of the aquarium. This study highlights how molecular tools like MLST can be used to investigate outbreaks of microbiological disease. This work reinforces the need for regular controls of water quality to reduce microbiological contamination due to waterborne microorganisms.",
author = "Guillaume Desoubeaux and Anne Debourgogne and Wiederhold, {Nathan P.} and Marie Zaffino and Deanna Sutton and Burns, {Rachel E.} and Salvatore Frasca and Hyatt, {Michael W.} and Carolyn Cray",
year = "2018",
month = "7",
day = "1",
doi = "10.1093/mmy/myx089",
language = "English (US)",
volume = "56",
pages = "591--601",
journal = "Medical Mycology",
issn = "1369-3786",
publisher = "Informa Healthcare",
number = "5",

}

TY - JOUR

T1 - Multi-locus sequence typing provides epidemiological insights for diseased sharks infected with fungi belonging to the Fusarium solani species complex

AU - Desoubeaux, Guillaume

AU - Debourgogne, Anne

AU - Wiederhold, Nathan P.

AU - Zaffino, Marie

AU - Sutton, Deanna

AU - Burns, Rachel E.

AU - Frasca, Salvatore

AU - Hyatt, Michael W.

AU - Cray, Carolyn

PY - 2018/7/1

Y1 - 2018/7/1

N2 - Fusarium spp. are saprobic moulds that are responsible for severe opportunistic infections in humans and animals. However, we need epidemiological tools to reliably trace the circulation of such fungal strains within medical or veterinary facilities, to recognize environmental contaminations that might lead to infection and to improve our understanding of factors responsible for the onset of outbreaks. In this study, we used molecular genotyping to investigate clustered cases of Fusarium solani species complex (FSSC) infection that occurred in eight Sphyrnidae sharks under managed care at a public aquarium. Genetic relationships between fungal strains were determined by multi-locus sequence typing (MLST) analysis based on DNA sequencing at five loci, followed by comparison with sequences of 50 epidemiologically unrelated FSSC strains. Our genotyping approach revealed that F. keratoplasticum and F. solani haplotype 9x were most commonly isolated. In one case, the infection proved to be with another Hypocrealian rare opportunistic pathogen Metarhizium robertsii. Twice, sharks proved to be infected with FSSC strains with the same MLST sequence type, supporting the hypothesis the hypothesis that common environmental populations of fungi existed for these sharks and would suggest the longtime persistence of the two clonal strains within the environment, perhaps in holding pools and life support systems of the aquarium. This study highlights how molecular tools like MLST can be used to investigate outbreaks of microbiological disease. This work reinforces the need for regular controls of water quality to reduce microbiological contamination due to waterborne microorganisms.

AB - Fusarium spp. are saprobic moulds that are responsible for severe opportunistic infections in humans and animals. However, we need epidemiological tools to reliably trace the circulation of such fungal strains within medical or veterinary facilities, to recognize environmental contaminations that might lead to infection and to improve our understanding of factors responsible for the onset of outbreaks. In this study, we used molecular genotyping to investigate clustered cases of Fusarium solani species complex (FSSC) infection that occurred in eight Sphyrnidae sharks under managed care at a public aquarium. Genetic relationships between fungal strains were determined by multi-locus sequence typing (MLST) analysis based on DNA sequencing at five loci, followed by comparison with sequences of 50 epidemiologically unrelated FSSC strains. Our genotyping approach revealed that F. keratoplasticum and F. solani haplotype 9x were most commonly isolated. In one case, the infection proved to be with another Hypocrealian rare opportunistic pathogen Metarhizium robertsii. Twice, sharks proved to be infected with FSSC strains with the same MLST sequence type, supporting the hypothesis the hypothesis that common environmental populations of fungi existed for these sharks and would suggest the longtime persistence of the two clonal strains within the environment, perhaps in holding pools and life support systems of the aquarium. This study highlights how molecular tools like MLST can be used to investigate outbreaks of microbiological disease. This work reinforces the need for regular controls of water quality to reduce microbiological contamination due to waterborne microorganisms.

UR - http://www.scopus.com/inward/record.url?scp=85052991868&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85052991868&partnerID=8YFLogxK

U2 - 10.1093/mmy/myx089

DO - 10.1093/mmy/myx089

M3 - Article

C2 - 29420818

AN - SCOPUS:85052991868

VL - 56

SP - 591

EP - 601

JO - Medical Mycology

JF - Medical Mycology

SN - 1369-3786

IS - 5

ER -