Muller glia stabilizes cell columns during retinal development

lateral cell migration but not neuropil growth is inhibited in mixed chick-quail retinospheroids

E. Willbold, M. Reinicke, C. Lance-Jones, C. Lagenaur, Vance Lemmon, P. G. Layer

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

Radial columnar organization of cell clones is a characteristic feature of vertebrate retinae that is structurally not understood. Here we provide in vitro evidence that Muller glia processes stabilize cells within columns. Dissociated embryonic chick retinal plus pigmented cells regenerate in vitro into fully laminated stratospheroids. After reaggregating chick and quail cells, quail-derived spheroid areas are detected as isolated sectors, as shown by a quail-specific antibody. Each sector contains one or multiple cell columns. The radial borders separating chick and quail sectors are fully congruent with the extension of 3A7-labelled Muller glia processes. While cell somata do not show any lateral interspecies mixing, quail-derived neuropil extends within the inner plexiform areas far into chick sectors. After selective damage of Muller cells by the gliotoxin DL-α-aminoadipic acid, the columnar organization is destabilized, as evidenced by a decrease in vimentin expression and by the migration of individual neurons out of their cell column. These data demonstrate that Muller cells actively stabilize cells within their columns, while neuritic growth is not hindered.

Original languageEnglish
Pages (from-to)2277-2284
Number of pages8
JournalEuropean Journal of Neuroscience
Volume7
Issue number11
DOIs
StatePublished - Nov 14 1995
Externally publishedYes

Fingerprint

Quail
Neuropil
Neuroglia
Cell Movement
Growth
Ependymoglial Cells
Gliotoxin
Carisoprodol
Vimentin
Vertebrates
Retina
Clone Cells
Neurons
Acids
Antibodies

Keywords

  • Chick
  • Differentiation
  • Fibre growth
  • Radial glia
  • Regeneration
  • Retinogenesis

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Muller glia stabilizes cell columns during retinal development : lateral cell migration but not neuropil growth is inhibited in mixed chick-quail retinospheroids. / Willbold, E.; Reinicke, M.; Lance-Jones, C.; Lagenaur, C.; Lemmon, Vance; Layer, P. G.

In: European Journal of Neuroscience, Vol. 7, No. 11, 14.11.1995, p. 2277-2284.

Research output: Contribution to journalArticle

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