Mucin production in the middle ear in response to lipopolysaccharides

S. E. Hunter, A. K. Singla, J. Prazma, B. S. Jewett, S. H. Randell, H. C. Pillsbury

Research output: Contribution to journalArticle

21 Scopus citations

Abstract

OBJECTIVE: This study examined the response of middle ear tissue to establish the lowest dose of lipopolysaccharide to induce mucin production in a rat otitis media model. METHODS: Twenty-six male Sprague-Dawley rats' eustachian tubes were obstructed before transtympanic inoculation of the bulla tympanica with 35 μL of Krebs Ringer or 1, 10, 100, or 1000 μg/mL lipopolysaccharide. After 7 days the effusion and a lavage were collected for mucin ELISA measurement, and tissue was collected for histologic evaluation. RESULTS: Mucin secretion was significantly increased in the 100 μg/mL 51.20 ± 13.6 μg/mL (SE) and 1000 μg/mL 69.42 ± 8.57 μg/mL groups when compared with the Krebs Ringer control group 1.84 ± 0.28 μg/mL (P < 0.05). Histologic evaluation shows goblet cell metaplasia and hyperplasia in the middle ear epithelium in the 1000 and 100 μg/mL groups. CONCLUSIONS: The histology and ELISA results suggest that a middle ear effusion is generated with a dose of lipopolysaccharide as low as 100 μg/mL.

Original languageEnglish (US)
Pages (from-to)884-888
Number of pages5
JournalOtolaryngology - Head and Neck Surgery
Volume120
Issue number6
DOIs
StatePublished - Jan 1 1999

ASJC Scopus subject areas

  • Surgery
  • Otorhinolaryngology

Fingerprint Dive into the research topics of 'Mucin production in the middle ear in response to lipopolysaccharides'. Together they form a unique fingerprint.

  • Cite this

    Hunter, S. E., Singla, A. K., Prazma, J., Jewett, B. S., Randell, S. H., & Pillsbury, H. C. (1999). Mucin production in the middle ear in response to lipopolysaccharides. Otolaryngology - Head and Neck Surgery, 120(6), 884-888. https://doi.org/10.1016/S0194-5998(99)70331-1