Mucin production in the middle ear in response to lipopolysaccharides

S. E. Hunter, A. K. Singla, J. Prazma, B. S. Jewett, S. H. Randell, H. C. Pillsbury

Research output: Contribution to journalArticlepeer-review

21 Scopus citations


OBJECTIVE: This study examined the response of middle ear tissue to establish the lowest dose of lipopolysaccharide to induce mucin production in a rat otitis media model. METHODS: Twenty-six male Sprague-Dawley rats' eustachian tubes were obstructed before transtympanic inoculation of the bulla tympanica with 35 μL of Krebs Ringer or 1, 10, 100, or 1000 μg/mL lipopolysaccharide. After 7 days the effusion and a lavage were collected for mucin ELISA measurement, and tissue was collected for histologic evaluation. RESULTS: Mucin secretion was significantly increased in the 100 μg/mL 51.20 ± 13.6 μg/mL (SE) and 1000 μg/mL 69.42 ± 8.57 μg/mL groups when compared with the Krebs Ringer control group 1.84 ± 0.28 μg/mL (P < 0.05). Histologic evaluation shows goblet cell metaplasia and hyperplasia in the middle ear epithelium in the 1000 and 100 μg/mL groups. CONCLUSIONS: The histology and ELISA results suggest that a middle ear effusion is generated with a dose of lipopolysaccharide as low as 100 μg/mL.

Original languageEnglish (US)
Pages (from-to)884-888
Number of pages5
JournalOtolaryngology - Head and Neck Surgery
Issue number6
StatePublished - 1999

ASJC Scopus subject areas

  • Surgery
  • Otorhinolaryngology


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