MUC4 (sialomucin complex) expression in salivary gland tumors and squamous cell carcinoma of the upper aerodigestive tract

Donald Weed, Carmen Gomez-Fernandez, Esteban Bonfante, Thomas D. Lee, Jeffrey Pacheco, Maria E. Carvajal, W. Jarrard Goodwin, Kermit L. Carraway

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

OBJECTIVES: This study investigates MUC4 expression in normal squamous epithelia and squamous cell carcinoma (SCC) of the upper aerodigestive tract (UADT), and in salivary gland neoplasms. STUDY DESIGN: MUC4 antigens in tumor and adjacent normal tissue are localized by immunocytochemical studies. Fresh frozen tissues from surgical resection specimens are further analyzed by Western blot. RESULTS: MUC4 is identified by immunocytochemical staining throughout the normal UADT mucosa, in 34 of 40 primary UADT SCC, and in 11 of 12 metastatic cervical lymph nodes. A trend toward decreased MUC4 staining in moderately and poorly differentiated tumors is noted. Immunoblots show MUC4 in 4 of 5 SCC analyzed. Immunocytochemical staining of MUC4 in 13 major and minor salivary gland neoplasms reveal variable staining of normal and neoplastic tissue. MUC4 is demonstrated in immunoblots of normal parotid tissue and in the single parotid malignancy analyzed, but is not demonstrated in one minor salivary gland malignancy. These findings characterize normal UADT mucosal and salivary MUC4 expression, and MUC4 expression in SCC of the UADT and in salivary gland tumors. SIGNIFICANCE: Correlation of MUC4 expression with clinical outcomes may establish MUC4 as a potential molecular prognostic marker for these tumors.

Original languageEnglish
Pages (from-to)127-141
Number of pages15
JournalOtolaryngology - Head and Neck Surgery
Volume124
Issue number2
DOIs
StatePublished - Mar 3 2001

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Sialomucins
Glandular and Epithelial Neoplasms
Squamous Cell Carcinoma
Salivary Gland Neoplasms
Staining and Labeling
Minor Salivary Glands
Far-Western Blotting
Neoplasms
Neoplasm Antigens
Tumor Biomarkers
Mucous Membrane
Epithelium
Lymph Nodes

ASJC Scopus subject areas

  • Otorhinolaryngology

Cite this

MUC4 (sialomucin complex) expression in salivary gland tumors and squamous cell carcinoma of the upper aerodigestive tract. / Weed, Donald; Gomez-Fernandez, Carmen; Bonfante, Esteban; Lee, Thomas D.; Pacheco, Jeffrey; Carvajal, Maria E.; Goodwin, W. Jarrard; Carraway, Kermit L.

In: Otolaryngology - Head and Neck Surgery, Vol. 124, No. 2, 03.03.2001, p. 127-141.

Research output: Contribution to journalArticle

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AU - Lee, Thomas D.

AU - Pacheco, Jeffrey

AU - Carvajal, Maria E.

AU - Goodwin, W. Jarrard

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