MTG1 codes for a conserved protein required for mitochondrial translation

Antonio Barrientos, Daniel Korr, Karen J. Barwell, Christian Sjulsen, Carl D. Gajewski, Giovanni Manfredi, Sharon Ackerman, Alexander Tzagoloff

Research output: Contribution to journalArticle

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Abstract

The MTG1 gene of Saccharomyces cerevisiae, corresponding to ORF YMR097c on chromosome XIII, codes for a mitochondrial protein essential for respiratory competence. A human homologue of Mtg1p capable of partially rescuing the respiratory deficiency of a yeast mtg1 mutant has also been localized in mitochondria. Mtg1p is a member of a family of GTPases with largely unknown functions. The respiratory deficiency of mtg1 mutants stems from a defect in mitochondrial protein synthesis. Mutations in the 21S rRNA locus are able to suppress the translation defect of mtg1 null mutants. This points to the 21S rRNA or the large ribosomal subunit as the most likely target of Mtg1p action. The presence of mature size 15S and 21S mitochondrial rRNAs in mtg1 mutants excludes Mtg1p from being involved in transcription or processing of these RNAs. More likely, Mtg1p functions in assembly of the large ribosomal subunit. This is consistent with the peripheral localization of Mtg1p on the matrix side of the inner membrane and the results of in vivo mitochondrial translation assays in a temperature-sensitive mtg1 mutant.

Original languageEnglish
Pages (from-to)2292-2302
Number of pages11
JournalMolecular Biology of the Cell
Volume14
Issue number6
DOIs
StatePublished - Jun 1 2003

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Mitochondrial Proteins
Large Ribosome Subunits
GTP Phosphohydrolases
Mental Competency
Open Reading Frames
Saccharomyces cerevisiae
Mitochondria
Chromosomes
Yeasts
RNA
Mutation
Temperature
Membranes
Genes
21S rRNA

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

Barrientos, A., Korr, D., Barwell, K. J., Sjulsen, C., Gajewski, C. D., Manfredi, G., ... Tzagoloff, A. (2003). MTG1 codes for a conserved protein required for mitochondrial translation. Molecular Biology of the Cell, 14(6), 2292-2302. https://doi.org/10.1091/mbc.E02-10-0636

MTG1 codes for a conserved protein required for mitochondrial translation. / Barrientos, Antonio; Korr, Daniel; Barwell, Karen J.; Sjulsen, Christian; Gajewski, Carl D.; Manfredi, Giovanni; Ackerman, Sharon; Tzagoloff, Alexander.

In: Molecular Biology of the Cell, Vol. 14, No. 6, 01.06.2003, p. 2292-2302.

Research output: Contribution to journalArticle

Barrientos, A, Korr, D, Barwell, KJ, Sjulsen, C, Gajewski, CD, Manfredi, G, Ackerman, S & Tzagoloff, A 2003, 'MTG1 codes for a conserved protein required for mitochondrial translation', Molecular Biology of the Cell, vol. 14, no. 6, pp. 2292-2302. https://doi.org/10.1091/mbc.E02-10-0636
Barrientos, Antonio ; Korr, Daniel ; Barwell, Karen J. ; Sjulsen, Christian ; Gajewski, Carl D. ; Manfredi, Giovanni ; Ackerman, Sharon ; Tzagoloff, Alexander. / MTG1 codes for a conserved protein required for mitochondrial translation. In: Molecular Biology of the Cell. 2003 ; Vol. 14, No. 6. pp. 2292-2302.
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