Mss51 and Ssc1 facilitate translational regulation of cytochrome c oxidase biogenesis

Flavia Fontanesi, Iliana C. Soto, Darryl Horn, Antoni Barrientos

Research output: Contribution to journalArticle

57 Scopus citations

Abstract

The intricate biogenesis of multimeric organellar enzymes of dual genetic origin entails several levels of regulation. In Saccharomyces cerevisiae, mitochondrial cytochrome c oxidase (COX) assembly is regulated translationally. Synthesis of subunit 1 (Cox1) is contingent on the availability of its assembly partners, thereby acting as a negative feedback loop that coordinates COX1 mRNA translation with Cox1 utilization during COX assembly. The COX1 mRNA-specific translational activator Mss51 plays a fundamental role in this process. Here, we report that Mss51 successively interacts with the COX1 mRNA translational apparatus, newly synthesized Cox1, and other COX assembly factors during Cox1 maturation/assembly. Notably, the mitochondrial Hsp70 chaperone Ssc1 is shown to be an Mss51 partner throughout its metabolic cycle. We conclude that Ssc1, by interacting with Mss51 and Mss51-containing complexes, plays a critical role in Cox1 biogenesis, COX assembly, and the translational regulation of these processes.

Original languageEnglish (US)
Pages (from-to)245-259
Number of pages15
JournalMolecular and cellular biology
Volume30
Issue number1
DOIs
StatePublished - Jan 2010

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

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