MRD detection in multiple myeloma: Comparison between MSKCC 10-color single-tube and EuroFlow 8-color 2-tube methods

Mikhail Roshal, Juan A. Flores-Montero, Qi Gao, Maesa Koeber, Jessica Wardrope, Brian G.M. Durie, Ahmet Dogan, Alberto Orfao, Ola Landgren

Research output: Contribution to journalArticlepeer-review

61 Scopus citations

Abstract

In patients with multiple myeloma, obtaining posttreatment minimal residual disease (MRD) negativity is associated with longer progression-free survival and overall survival. Here, we compared the diagnostic performance of a single 10-color tube with that of a EuroFlow 8-color 2-tube panel for MRD testing. Bone marrow samples from 41 multiple myeloma patients were tested in parallel using the 2 approaches. Compared with the sum of the cells from the EuroFlow two 8-color tubes, the Memorial Sloan Kettering Cancer Center (MSKCC) single 10-color tube had a slight reduction in total cell number with a mean ratio of 0.85 (range, 0.57-1.46; P, .05), likely attributable to permeabilization of the cells. Percent of plasma cells showed a high degree of concordance (r2 5 0.97) as did normal plasma cells (r2 5 0.96), consistent with no selective plasma cell loss. Importantly, concordant measurement of residual disease burden was seen with abnormal plasma cells (r2 5 0.97). The overall concordance between the 2 tests was 98%. In 1 case, there was a discrepancy near the limit of detection of both tests in favor of the slightly greater theoretical sensitivity of the EuroFlow 8-color 2-tube panel (analytical sensitivity limit of MSKCC single 10-color tube: 6 cells in 1 million with at least 3 million cell acquisitions; EuroFlow 8-color 2-tube panel: 2 cells in 1 million with the recommended 10 million cell acquisitions).

Original languageEnglish (US)
Pages (from-to)728-732
Number of pages5
JournalBlood Advances
Volume1
Issue number12
DOIs
StatePublished - May 9 2017
Externally publishedYes

ASJC Scopus subject areas

  • Hematology

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