Mouse taste buds use serotonin as a neurotransmitter

Yi Jen Huang, Yutaka Maruyama, Kuo Shyan Lu, Elizabeth Pereira, Ilya Plonsky, John E. Baur, Dianqing Wu, Stephen D Roper

Research output: Contribution to journalArticle

137 Citations (Scopus)

Abstract

Synapses between gustatory receptor cells and primary sensory afferent fibers transmit the output signal from taste buds to the CNS. Several transmitter candidates have been proposed for these synapses, including serotonin (5-HT), glutamate, acetylcholine, ATP, peptides, and others, but, to date, none has been unambiguously identified. We used Chinese hamster ovary cells stably expressing 5-HT2C receptors as biodetectors to monitor 5-HT release from taste buds. When taste buds were depolarized with KCl or stimulated with bitter, sweet, or sour (acid) tastants, serotonin was released. KCl- and acid-induced 5-HT release, but not release attributable to sweet or bitter stimulation, required Ca2+ influx. In contrast, 5-HT release evoked by sweet and bitter stimulation seemed to be triggered by intracellular Ca2+ release. These experiments strongly implicate serotonin as a taste bud neurotransmitter and reveal unexpected transmitter release mechanisms.

Original languageEnglish
Pages (from-to)843-847
Number of pages5
JournalJournal of Neuroscience
Volume25
Issue number4
DOIs
StatePublished - Feb 26 2005

Fingerprint

Taste Buds
Neurotransmitter Agents
Serotonin
Synapses
Receptor, Serotonin, 5-HT2C
Acids
Sensory Receptor Cells
Cricetulus
Acetylcholine
Glutamic Acid
Ovary
Adenosine Triphosphate
Peptides

Keywords

  • Biosensors
  • Calcium imaging
  • Phospholipase C β2
  • Serotonin
  • Taste
  • Transmitters

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Huang, Y. J., Maruyama, Y., Lu, K. S., Pereira, E., Plonsky, I., Baur, J. E., ... Roper, S. D. (2005). Mouse taste buds use serotonin as a neurotransmitter. Journal of Neuroscience, 25(4), 843-847. https://doi.org/10.1523/JNEUROSCI.4446-04.2005

Mouse taste buds use serotonin as a neurotransmitter. / Huang, Yi Jen; Maruyama, Yutaka; Lu, Kuo Shyan; Pereira, Elizabeth; Plonsky, Ilya; Baur, John E.; Wu, Dianqing; Roper, Stephen D.

In: Journal of Neuroscience, Vol. 25, No. 4, 26.02.2005, p. 843-847.

Research output: Contribution to journalArticle

Huang, YJ, Maruyama, Y, Lu, KS, Pereira, E, Plonsky, I, Baur, JE, Wu, D & Roper, SD 2005, 'Mouse taste buds use serotonin as a neurotransmitter', Journal of Neuroscience, vol. 25, no. 4, pp. 843-847. https://doi.org/10.1523/JNEUROSCI.4446-04.2005
Huang YJ, Maruyama Y, Lu KS, Pereira E, Plonsky I, Baur JE et al. Mouse taste buds use serotonin as a neurotransmitter. Journal of Neuroscience. 2005 Feb 26;25(4):843-847. https://doi.org/10.1523/JNEUROSCI.4446-04.2005
Huang, Yi Jen ; Maruyama, Yutaka ; Lu, Kuo Shyan ; Pereira, Elizabeth ; Plonsky, Ilya ; Baur, John E. ; Wu, Dianqing ; Roper, Stephen D. / Mouse taste buds use serotonin as a neurotransmitter. In: Journal of Neuroscience. 2005 ; Vol. 25, No. 4. pp. 843-847.
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