Mouse ret finger protein (rfp) proto-oncogene is expressed at specific stages of mouse spermatogenesis

Tongyu Wikramanayake, Mark Shannon, Mary Ann Handel, Laurence D. Etkin

Research output: Contribution to journalArticle

44 Citations (Scopus)

Abstract

Many proteins involved in the regulation of cell growth and differentiation possess structural motifs that participate in specific molecular interactions. The human rfp (ret finger protein) has a tripartite motif, consisting of two novel zinc fingers (the RING finger and the B box) and a coiled-coil domain, and belongs to the B box zinc finger protein family. Rfp becomes oncogenic when its tripartite motif is recombined with the tyrosine kinase domain from the cret proto-oncogene. To further understand the function of rfp during normal development and cellular differentiation, we cloned the mouse rfp cDNA and analyzed its pattern of expression and subcellular distribution. We found that the mouse rfp cDNA shared a 98.4% homology with the human sequence. The gene mapped to human chromosome 6 and mouse chromosome 13 indicating that it was linked to a several other genes encoding proteins that possess common domains. rfp transcripts and protein were ubiquitous in day 10.5-13.5 mouse embryos; however, they were restricted in adult mice, with the highest level of expression in pachytene spermatocytes and round spermatids of differentiating sperm. The rfp protein was detected within cell nuclei as nuclear bodies similar to the PODs (PML oncogenic domains) observed with another B box family member. PML (promyelocytic leukemia protein). These results suggest that rfp may function in the regulation of cell growth and differentiation during mouse embryogenesis and sperm differentiation.

Original languageEnglish
Pages (from-to)309-320
Number of pages12
JournalDevelopmental Genetics
Volume19
Issue number4
DOIs
StatePublished - Dec 1 1996
Externally publishedYes

Fingerprint

Proto-Oncogenes
Spermatogenesis
Proteins
Fingers
Zinc Fingers
Spermatozoa
Cell Differentiation
Complementary DNA
Mouse Trim27 protein
Chromosomes, Human, Pair 13
Chromosomes, Human, Pair 6
Spermatocytes
Spermatids
Human Chromosomes
Growth
Cell Nucleus
Protein-Tyrosine Kinases
Embryonic Development
Embryonic Structures

Keywords

  • B box
  • expression
  • ret finger protein
  • RING finger

ASJC Scopus subject areas

  • Developmental Biology
  • Cell Biology
  • Genetics

Cite this

Mouse ret finger protein (rfp) proto-oncogene is expressed at specific stages of mouse spermatogenesis. / Wikramanayake, Tongyu; Shannon, Mark; Handel, Mary Ann; Etkin, Laurence D.

In: Developmental Genetics, Vol. 19, No. 4, 01.12.1996, p. 309-320.

Research output: Contribution to journalArticle

Wikramanayake, Tongyu ; Shannon, Mark ; Handel, Mary Ann ; Etkin, Laurence D. / Mouse ret finger protein (rfp) proto-oncogene is expressed at specific stages of mouse spermatogenesis. In: Developmental Genetics. 1996 ; Vol. 19, No. 4. pp. 309-320.
@article{bb5145fd26f84d239abd52394d0536b0,
title = "Mouse ret finger protein (rfp) proto-oncogene is expressed at specific stages of mouse spermatogenesis",
abstract = "Many proteins involved in the regulation of cell growth and differentiation possess structural motifs that participate in specific molecular interactions. The human rfp (ret finger protein) has a tripartite motif, consisting of two novel zinc fingers (the RING finger and the B box) and a coiled-coil domain, and belongs to the B box zinc finger protein family. Rfp becomes oncogenic when its tripartite motif is recombined with the tyrosine kinase domain from the cret proto-oncogene. To further understand the function of rfp during normal development and cellular differentiation, we cloned the mouse rfp cDNA and analyzed its pattern of expression and subcellular distribution. We found that the mouse rfp cDNA shared a 98.4{\%} homology with the human sequence. The gene mapped to human chromosome 6 and mouse chromosome 13 indicating that it was linked to a several other genes encoding proteins that possess common domains. rfp transcripts and protein were ubiquitous in day 10.5-13.5 mouse embryos; however, they were restricted in adult mice, with the highest level of expression in pachytene spermatocytes and round spermatids of differentiating sperm. The rfp protein was detected within cell nuclei as nuclear bodies similar to the PODs (PML oncogenic domains) observed with another B box family member. PML (promyelocytic leukemia protein). These results suggest that rfp may function in the regulation of cell growth and differentiation during mouse embryogenesis and sperm differentiation.",
keywords = "B box, expression, ret finger protein, RING finger",
author = "Tongyu Wikramanayake and Mark Shannon and Handel, {Mary Ann} and Etkin, {Laurence D.}",
year = "1996",
month = "12",
day = "1",
doi = "10.1002/(SICI)1520-6408(1996)19:4<309::AID-DVG4>3.0.CO;2-D",
language = "English",
volume = "19",
pages = "309--320",
journal = "Genesis",
issn = "1526-954X",
publisher = "Wiley-Liss Inc.",
number = "4",

}

TY - JOUR

T1 - Mouse ret finger protein (rfp) proto-oncogene is expressed at specific stages of mouse spermatogenesis

AU - Wikramanayake, Tongyu

AU - Shannon, Mark

AU - Handel, Mary Ann

AU - Etkin, Laurence D.

PY - 1996/12/1

Y1 - 1996/12/1

N2 - Many proteins involved in the regulation of cell growth and differentiation possess structural motifs that participate in specific molecular interactions. The human rfp (ret finger protein) has a tripartite motif, consisting of two novel zinc fingers (the RING finger and the B box) and a coiled-coil domain, and belongs to the B box zinc finger protein family. Rfp becomes oncogenic when its tripartite motif is recombined with the tyrosine kinase domain from the cret proto-oncogene. To further understand the function of rfp during normal development and cellular differentiation, we cloned the mouse rfp cDNA and analyzed its pattern of expression and subcellular distribution. We found that the mouse rfp cDNA shared a 98.4% homology with the human sequence. The gene mapped to human chromosome 6 and mouse chromosome 13 indicating that it was linked to a several other genes encoding proteins that possess common domains. rfp transcripts and protein were ubiquitous in day 10.5-13.5 mouse embryos; however, they were restricted in adult mice, with the highest level of expression in pachytene spermatocytes and round spermatids of differentiating sperm. The rfp protein was detected within cell nuclei as nuclear bodies similar to the PODs (PML oncogenic domains) observed with another B box family member. PML (promyelocytic leukemia protein). These results suggest that rfp may function in the regulation of cell growth and differentiation during mouse embryogenesis and sperm differentiation.

AB - Many proteins involved in the regulation of cell growth and differentiation possess structural motifs that participate in specific molecular interactions. The human rfp (ret finger protein) has a tripartite motif, consisting of two novel zinc fingers (the RING finger and the B box) and a coiled-coil domain, and belongs to the B box zinc finger protein family. Rfp becomes oncogenic when its tripartite motif is recombined with the tyrosine kinase domain from the cret proto-oncogene. To further understand the function of rfp during normal development and cellular differentiation, we cloned the mouse rfp cDNA and analyzed its pattern of expression and subcellular distribution. We found that the mouse rfp cDNA shared a 98.4% homology with the human sequence. The gene mapped to human chromosome 6 and mouse chromosome 13 indicating that it was linked to a several other genes encoding proteins that possess common domains. rfp transcripts and protein were ubiquitous in day 10.5-13.5 mouse embryos; however, they were restricted in adult mice, with the highest level of expression in pachytene spermatocytes and round spermatids of differentiating sperm. The rfp protein was detected within cell nuclei as nuclear bodies similar to the PODs (PML oncogenic domains) observed with another B box family member. PML (promyelocytic leukemia protein). These results suggest that rfp may function in the regulation of cell growth and differentiation during mouse embryogenesis and sperm differentiation.

KW - B box

KW - expression

KW - ret finger protein

KW - RING finger

UR - http://www.scopus.com/inward/record.url?scp=0030482030&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0030482030&partnerID=8YFLogxK

U2 - 10.1002/(SICI)1520-6408(1996)19:4<309::AID-DVG4>3.0.CO;2-D

DO - 10.1002/(SICI)1520-6408(1996)19:4<309::AID-DVG4>3.0.CO;2-D

M3 - Article

C2 - 9023983

AN - SCOPUS:0030482030

VL - 19

SP - 309

EP - 320

JO - Genesis

JF - Genesis

SN - 1526-954X

IS - 4

ER -