Mouse model of touch-evoked itch (alloknesis)

Tasuku Akiyama, Mirela Iodi Carstens, Akihiko Ikoma, Ferda Cevikbas, Martin Steinhoff, Earl Carstens

Research output: Contribution to journalArticle

50 Citations (Scopus)

Abstract

Lightly touching normal skin near a site of itch can elicit itch sensation, a phenomenon known as alloknesis. To investigate the neural mechanisms of alloknesis, we have developed an animal model. Low-threshold mechanical stimulation of the skin normally does not elicit any response in naive C57/BL6 mice. Following acute intradermal (i.d.) injection of histamine in the rostral back, mechanical stimulation 7 mm from the injection site elicited discrete hindlimb scratch bouts directed toward the stimulus. This began at 10 minutes and peaked 20-40 minutes post histamine injection, declining over the next hour. Histamine itself elicited bouts of scratching not associated with the mechanical stimulus, which ceased after 30 minutes. Histamine- and touch-evoked scratching was inhibited by the μ-opiate antagonist naltrexone. Touch-evoked scratching was observed following i.d. 5-HT (5-hydroxytryptamine), a protease-activated receptor (PAR)-4 agonist, and an MrgprC11 agonist BAM8-22, but not chloroquine or a PAR-2 agonist. The histamine H1 receptor antagonist terfenadine prevented scratching and alloknesis evoked by histamine, but not 5-HT, a PAR-4 agonist or an MrgprC11 agonist. In mice with experimental dry skin, there was a time-dependent increase in spontaneous and touch-evoked scratching. This animal model appears to be useful to investigate neural mechanisms of itch and alloknesis.

Original languageEnglish (US)
Pages (from-to)1886-1891
Number of pages6
JournalJournal of Investigative Dermatology
Volume132
Issue number7
DOIs
StatePublished - Jan 1 2012
Externally publishedYes

Fingerprint

Touch
Histamine
Skin
Opiate Alkaloids
Serotonin
Animals
Animal Models
PAR-2 Receptor
Terfenadine
Histamine H1 Antagonists
Intradermal Injections
Naltrexone
Injections
Chloroquine
Hindlimb
protease-activated receptor 4

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Dermatology
  • Cell Biology

Cite this

Akiyama, T., Carstens, M. I., Ikoma, A., Cevikbas, F., Steinhoff, M., & Carstens, E. (2012). Mouse model of touch-evoked itch (alloknesis). Journal of Investigative Dermatology, 132(7), 1886-1891. https://doi.org/10.1038/jid.2012.52

Mouse model of touch-evoked itch (alloknesis). / Akiyama, Tasuku; Carstens, Mirela Iodi; Ikoma, Akihiko; Cevikbas, Ferda; Steinhoff, Martin; Carstens, Earl.

In: Journal of Investigative Dermatology, Vol. 132, No. 7, 01.01.2012, p. 1886-1891.

Research output: Contribution to journalArticle

Akiyama, T, Carstens, MI, Ikoma, A, Cevikbas, F, Steinhoff, M & Carstens, E 2012, 'Mouse model of touch-evoked itch (alloknesis)', Journal of Investigative Dermatology, vol. 132, no. 7, pp. 1886-1891. https://doi.org/10.1038/jid.2012.52
Akiyama T, Carstens MI, Ikoma A, Cevikbas F, Steinhoff M, Carstens E. Mouse model of touch-evoked itch (alloknesis). Journal of Investigative Dermatology. 2012 Jan 1;132(7):1886-1891. https://doi.org/10.1038/jid.2012.52
Akiyama, Tasuku ; Carstens, Mirela Iodi ; Ikoma, Akihiko ; Cevikbas, Ferda ; Steinhoff, Martin ; Carstens, Earl. / Mouse model of touch-evoked itch (alloknesis). In: Journal of Investigative Dermatology. 2012 ; Vol. 132, No. 7. pp. 1886-1891.
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