Abstract
Animal models of central nervous system (CNS) diseases and, consequently, blood-brain barrier disruption diseases, require the delivery of exogenous substances into the brain. These exogenous substances may induce injurious impact or constitute therapeutic strategy. The most common delivery methods of exogenous substances into the brain are based on systemic deliveries, such as subcutaneous or intravenous routes. Although commonly used, these approaches have several limitations, including low delivery efficacy into the brain. In contrast, surgical methods that locally deliver substances into the CNS are more specific and prevent the uptake of the exogenous substances by other organs. Several surgical methods for CNS delivery are available; however, they tend to be very traumatic. Here, we describe a mouse infusion microsurgery technique, which effectively delivers substances into the brain via the internal carotid artery, with minimal trauma and no interference with normal CNS functionality.
| Original language | English (US) |
|---|---|
| Article number | e54804 |
| Journal | Journal of Visualized Experiments |
| Volume | 2017 |
| Issue number | 119 |
| DOIs | |
| State | Published - Jan 31 2017 |
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Keywords
- CNS substance delivery
- Common carotid artery
- Exosomes
- External carotid artery
- HIV
- Internal carotid artery
- Issue 119
- Microsurgery
- Mouse
- Neuroscience
ASJC Scopus subject areas
- Neuroscience(all)
- Chemical Engineering(all)
- Immunology and Microbiology(all)
- Biochemistry, Genetics and Molecular Biology(all)
Cite this
Mouse microsurgery infusion technique for targeted substance delivery into the CNS via the internal carotid artery. / Leda, Ana R.; Dygert, Levy; Bertrand, Luc; Toborek, Michal J.
In: Journal of Visualized Experiments, Vol. 2017, No. 119, e54804, 31.01.2017.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Mouse microsurgery infusion technique for targeted substance delivery into the CNS via the internal carotid artery
AU - Leda, Ana R.
AU - Dygert, Levy
AU - Bertrand, Luc
AU - Toborek, Michal J
PY - 2017/1/31
Y1 - 2017/1/31
N2 - Animal models of central nervous system (CNS) diseases and, consequently, blood-brain barrier disruption diseases, require the delivery of exogenous substances into the brain. These exogenous substances may induce injurious impact or constitute therapeutic strategy. The most common delivery methods of exogenous substances into the brain are based on systemic deliveries, such as subcutaneous or intravenous routes. Although commonly used, these approaches have several limitations, including low delivery efficacy into the brain. In contrast, surgical methods that locally deliver substances into the CNS are more specific and prevent the uptake of the exogenous substances by other organs. Several surgical methods for CNS delivery are available; however, they tend to be very traumatic. Here, we describe a mouse infusion microsurgery technique, which effectively delivers substances into the brain via the internal carotid artery, with minimal trauma and no interference with normal CNS functionality.
AB - Animal models of central nervous system (CNS) diseases and, consequently, blood-brain barrier disruption diseases, require the delivery of exogenous substances into the brain. These exogenous substances may induce injurious impact or constitute therapeutic strategy. The most common delivery methods of exogenous substances into the brain are based on systemic deliveries, such as subcutaneous or intravenous routes. Although commonly used, these approaches have several limitations, including low delivery efficacy into the brain. In contrast, surgical methods that locally deliver substances into the CNS are more specific and prevent the uptake of the exogenous substances by other organs. Several surgical methods for CNS delivery are available; however, they tend to be very traumatic. Here, we describe a mouse infusion microsurgery technique, which effectively delivers substances into the brain via the internal carotid artery, with minimal trauma and no interference with normal CNS functionality.
KW - CNS substance delivery
KW - Common carotid artery
KW - Exosomes
KW - External carotid artery
KW - HIV
KW - Internal carotid artery
KW - Issue 119
KW - Microsurgery
KW - Mouse
KW - Neuroscience
UR - http://www.scopus.com/inward/record.url?scp=85011601294&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85011601294&partnerID=8YFLogxK
U2 - 10.3791/54804
DO - 10.3791/54804
M3 - Article
C2 - 28190068
AN - SCOPUS:85011601294
VL - 2017
JO - Journal of visualized experiments : JoVE
JF - Journal of visualized experiments : JoVE
SN - 1940-087X
IS - 119
M1 - e54804
ER -