Mouse microsurgery infusion technique for targeted substance delivery into the CNS via the internal carotid artery

Ana R. Leda, Levy Dygert, Luc Bertrand, Michal J Toborek

Research output: Contribution to journalArticle

4 Scopus citations

Abstract

Animal models of central nervous system (CNS) diseases and, consequently, blood-brain barrier disruption diseases, require the delivery of exogenous substances into the brain. These exogenous substances may induce injurious impact or constitute therapeutic strategy. The most common delivery methods of exogenous substances into the brain are based on systemic deliveries, such as subcutaneous or intravenous routes. Although commonly used, these approaches have several limitations, including low delivery efficacy into the brain. In contrast, surgical methods that locally deliver substances into the CNS are more specific and prevent the uptake of the exogenous substances by other organs. Several surgical methods for CNS delivery are available; however, they tend to be very traumatic. Here, we describe a mouse infusion microsurgery technique, which effectively delivers substances into the brain via the internal carotid artery, with minimal trauma and no interference with normal CNS functionality.

Original languageEnglish (US)
Article numbere54804
JournalJournal of Visualized Experiments
Volume2017
Issue number119
DOIs
StatePublished - Jan 31 2017

Keywords

  • CNS substance delivery
  • Common carotid artery
  • Exosomes
  • External carotid artery
  • HIV
  • Internal carotid artery
  • Issue 119
  • Microsurgery
  • Mouse
  • Neuroscience

ASJC Scopus subject areas

  • Neuroscience(all)
  • Chemical Engineering(all)
  • Immunology and Microbiology(all)
  • Biochemistry, Genetics and Molecular Biology(all)

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