Mouse glomerular endothelial cells have an insulin receptor

Sharon Elliot, F. G. Conti, L. J. Striker, G. E. Striker

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

An insulin receptor was found on the surface of cloned mouse glomerular endothelial cells in vitro. Total specific binding was 2.5 ± 0.3%/106 cells at 90 min and 22°C. Analysis according to Scatchard resulted in a curvilinear plot, with a kd for the high and low affinity sites estimated at 1.41 x 10-10 and 8.2 x 10-8 respectively. Insulin binding decreased following 12 hour exposure to 50 ng/ml of insulin suggesting that down regulation of the receptor had occurred, an effect which was reversible. Covalent crosslinking of the receptor to 125I insulin revealed one band at M(r) 125,000 by SDS-PAGE which disappeared following preincubation with excess unlabeled insulin. Insulin was also able to stimulate phosphorylation of the β subunit. The characteristics of this insulin receptor appear very similar to that of endothelial cell types from other microvascular beds.

Original languageEnglish
Pages (from-to)557-560
Number of pages4
JournalHormone and Metabolic Research
Volume22
Issue number11
StatePublished - Dec 5 1990
Externally publishedYes

Fingerprint

Insulin Receptor
Endothelial cells
Endothelial Cells
Insulin
Phosphorylation
Crosslinking
Polyacrylamide Gel Electrophoresis
Down-Regulation

Keywords

  • crosslinking
  • downregulation
  • glomerular endothelium
  • phosphorylation
  • receptor

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology

Cite this

Elliot, S., Conti, F. G., Striker, L. J., & Striker, G. E. (1990). Mouse glomerular endothelial cells have an insulin receptor. Hormone and Metabolic Research, 22(11), 557-560.

Mouse glomerular endothelial cells have an insulin receptor. / Elliot, Sharon; Conti, F. G.; Striker, L. J.; Striker, G. E.

In: Hormone and Metabolic Research, Vol. 22, No. 11, 05.12.1990, p. 557-560.

Research output: Contribution to journalArticle

Elliot, S, Conti, FG, Striker, LJ & Striker, GE 1990, 'Mouse glomerular endothelial cells have an insulin receptor', Hormone and Metabolic Research, vol. 22, no. 11, pp. 557-560.
Elliot S, Conti FG, Striker LJ, Striker GE. Mouse glomerular endothelial cells have an insulin receptor. Hormone and Metabolic Research. 1990 Dec 5;22(11):557-560.
Elliot, Sharon ; Conti, F. G. ; Striker, L. J. ; Striker, G. E. / Mouse glomerular endothelial cells have an insulin receptor. In: Hormone and Metabolic Research. 1990 ; Vol. 22, No. 11. pp. 557-560.
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