Motoneuron replacement for reinnervation of skeletal muscle in adult rats

Robert M. Grumbles, Vania W. Almeida, Gizelda T B Casella, Patrick Wood, Kamondanai Hemstapat, Christine K Thomas

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Reinnervation is needed to rescue muscle when motoneurons die in disease or injury. Embryonic ventral spinal cord cells transplanted into peripheral nerve reinnervate muscle and reduce atrophy, but low motoneuron survival may limit motor unit formation. We tested whether transplantation of a purified population of embryonic motoneurons into peripheral nerve (mean ± SE, 146,458 ± 4,011 motoneurons) resulted in more motor units and reinnervation than transplantation of a mixed population of ventral spinal cord cells (72,075 ± 12,329 motoneurons). Ten weeks after either kind of transplant, similar numbers of neurons expressed choline acetyl transferase and/or Islet-1. Motoneuron numbers always exceeded the reinnervated motor unit count. Most motor end plate were simple plaques. Reinnervation significantly reduced muscle fiber atrophy. These data show that the number of transplanted motoneurons and motoneuron survival do not limit muscle reinnervation. Incomplete differentiation of motoneurons in nerve and lack of muscle activity may result in immature neuromuscular junctions that limit reinnervation and function.

Original languageEnglish
Pages (from-to)921-930
Number of pages10
JournalJournal of Neuropathology and Experimental Neurology
Volume71
Issue number10
DOIs
StatePublished - Oct 1 2012

Fingerprint

Motor Neurons
Skeletal Muscle
Muscular Atrophy
Peripheral Nerves
Muscles
Spinal Cord
Transplantation
Motor Endplate
Neuromuscular Junction
Transferases
Choline
Population
Transplants
Neurons
Wounds and Injuries

Keywords

  • Axon regeneration
  • Motoneuron transplantation
  • Motor unit
  • Muscle denervation
  • Muscle reinnervation
  • Neuromuscular junction

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Clinical Neurology
  • Neurology
  • Cellular and Molecular Neuroscience

Cite this

Grumbles, R. M., Almeida, V. W., Casella, G. T. B., Wood, P., Hemstapat, K., & Thomas, C. K. (2012). Motoneuron replacement for reinnervation of skeletal muscle in adult rats. Journal of Neuropathology and Experimental Neurology, 71(10), 921-930. https://doi.org/10.1097/NEN.0b013e31826cf69a

Motoneuron replacement for reinnervation of skeletal muscle in adult rats. / Grumbles, Robert M.; Almeida, Vania W.; Casella, Gizelda T B; Wood, Patrick; Hemstapat, Kamondanai; Thomas, Christine K.

In: Journal of Neuropathology and Experimental Neurology, Vol. 71, No. 10, 01.10.2012, p. 921-930.

Research output: Contribution to journalArticle

Grumbles, RM, Almeida, VW, Casella, GTB, Wood, P, Hemstapat, K & Thomas, CK 2012, 'Motoneuron replacement for reinnervation of skeletal muscle in adult rats', Journal of Neuropathology and Experimental Neurology, vol. 71, no. 10, pp. 921-930. https://doi.org/10.1097/NEN.0b013e31826cf69a
Grumbles, Robert M. ; Almeida, Vania W. ; Casella, Gizelda T B ; Wood, Patrick ; Hemstapat, Kamondanai ; Thomas, Christine K. / Motoneuron replacement for reinnervation of skeletal muscle in adult rats. In: Journal of Neuropathology and Experimental Neurology. 2012 ; Vol. 71, No. 10. pp. 921-930.
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