Abstract
Marked morphological responses occur in the gills of freshwater rainbow trout in response to experimental acid-base disturbance and these responses play an important role in acid-base correction. Compensated respiratory acidosis induced by 70h exposure to environmental hyperoxia (elevated water PO2) caused a 33% decrease in branchial chloride cell fractional surface area (CCFA). Metabolic alkalosis induced by normoxic recovery (6h) from hyperoxia (72h) caused a 50% increase in CCFA, whereas metabolic alkalosis induced by infusion (19h) of NaHCO3 caused a 70% rise. However, the largest increase (135%) in CCFA was seen in response to infusion (19h) of HCl. NaCl infusion had no effect. A particular goal was to assess the relative importance of changes in CCFA vs. changes in internal substrate (HCO3-) availability in regulating the activity of the branchial Cl-/HCO3- exchange system. For each of the experimental treatments, the accompanying blood acid-base status and branchial transport kinetics (Km, Jmax) for Cl- uptake had been determined in earlier studies. In the present study, a positive linear relationship was established between CCFA and JCl-max in individual control fish in the absence of an acid-base disturbance. By reference to this relationship, observed changes in JCl-max during metabolic acid-base disturbances were clearly due to changes in both CCFA and internal substrate levels (plasma [HCO3-]) with the two factors having approximately equal influence.
| Original language | English |
|---|---|
| Pages (from-to) | 465-477 |
| Number of pages | 13 |
| Journal | Fish Physiology and Biochemistry |
| Volume | 12 |
| Issue number | 6 |
| DOIs | |
| State | Published - Mar 1 1994 |
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Keywords
- acid-base
- chloride cell
- fish
- J
- K
- kinetics
- morphology
ASJC Scopus subject areas
- Physiology (medical)
- Physiology
- Biochemistry
- Biochemistry, Genetics and Molecular Biology(all)
- Aquatic Science
Cite this
Morphological responses of the rainbow trout (Oncorhynchus mykiss) gill to hyperoxia, base (NaHCO3) and acid (HCl) infusions. / Goss, Greg G.; Wood, Chris M.; Laurent, Pierre; Perry, Steve F.
In: Fish Physiology and Biochemistry, Vol. 12, No. 6, 01.03.1994, p. 465-477.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Morphological responses of the rainbow trout (Oncorhynchus mykiss) gill to hyperoxia, base (NaHCO3) and acid (HCl) infusions
AU - Goss, Greg G.
AU - Wood, Chris M.
AU - Laurent, Pierre
AU - Perry, Steve F.
PY - 1994/3/1
Y1 - 1994/3/1
N2 - Marked morphological responses occur in the gills of freshwater rainbow trout in response to experimental acid-base disturbance and these responses play an important role in acid-base correction. Compensated respiratory acidosis induced by 70h exposure to environmental hyperoxia (elevated water PO2) caused a 33% decrease in branchial chloride cell fractional surface area (CCFA). Metabolic alkalosis induced by normoxic recovery (6h) from hyperoxia (72h) caused a 50% increase in CCFA, whereas metabolic alkalosis induced by infusion (19h) of NaHCO3 caused a 70% rise. However, the largest increase (135%) in CCFA was seen in response to infusion (19h) of HCl. NaCl infusion had no effect. A particular goal was to assess the relative importance of changes in CCFA vs. changes in internal substrate (HCO3-) availability in regulating the activity of the branchial Cl-/HCO3- exchange system. For each of the experimental treatments, the accompanying blood acid-base status and branchial transport kinetics (Km, Jmax) for Cl- uptake had been determined in earlier studies. In the present study, a positive linear relationship was established between CCFA and JCl-max in individual control fish in the absence of an acid-base disturbance. By reference to this relationship, observed changes in JCl-max during metabolic acid-base disturbances were clearly due to changes in both CCFA and internal substrate levels (plasma [HCO3-]) with the two factors having approximately equal influence.
AB - Marked morphological responses occur in the gills of freshwater rainbow trout in response to experimental acid-base disturbance and these responses play an important role in acid-base correction. Compensated respiratory acidosis induced by 70h exposure to environmental hyperoxia (elevated water PO2) caused a 33% decrease in branchial chloride cell fractional surface area (CCFA). Metabolic alkalosis induced by normoxic recovery (6h) from hyperoxia (72h) caused a 50% increase in CCFA, whereas metabolic alkalosis induced by infusion (19h) of NaHCO3 caused a 70% rise. However, the largest increase (135%) in CCFA was seen in response to infusion (19h) of HCl. NaCl infusion had no effect. A particular goal was to assess the relative importance of changes in CCFA vs. changes in internal substrate (HCO3-) availability in regulating the activity of the branchial Cl-/HCO3- exchange system. For each of the experimental treatments, the accompanying blood acid-base status and branchial transport kinetics (Km, Jmax) for Cl- uptake had been determined in earlier studies. In the present study, a positive linear relationship was established between CCFA and JCl-max in individual control fish in the absence of an acid-base disturbance. By reference to this relationship, observed changes in JCl-max during metabolic acid-base disturbances were clearly due to changes in both CCFA and internal substrate levels (plasma [HCO3-]) with the two factors having approximately equal influence.
KW - acid-base
KW - chloride cell
KW - fish
KW - J
KW - K
KW - kinetics
KW - morphology
UR - http://www.scopus.com/inward/record.url?scp=0001633203&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0001633203&partnerID=8YFLogxK
U2 - 10.1007/BF00004449
DO - 10.1007/BF00004449
M3 - Article
C2 - 24203108
AN - SCOPUS:0001633203
VL - 12
SP - 465
EP - 477
JO - Fish Physiology and Biochemistry
JF - Fish Physiology and Biochemistry
SN - 0920-1742
IS - 6
ER -