Morphologic effects of ammonia on primary astrocyte cultures. II. Electron microscopic studies

J. B. Gregorios, L. W. Mozes, Michael D Norenberg

Research output: Contribution to journalArticle

78 Citations (Scopus)

Abstract

Light microscopic studies of primary astrocyte cultures following exposure to ammonia have shown several alterations. To determine the nature and significance of these changes, electron microscopic studies were performed. Ultrastructural changes consisted of proliferation, pleomorphism and swelling of mitochondria, condensation of the mitochondrial matrix, cytoplasmic lucency and vacuolization, disaggregation of polyribosomal clusters, an initial increase followed by degranulation of rough endoplasmic reticulum, proliferation of smooth endoplasmic reticulum, an accumulation of dense bodies and a loss of intermediate glial filaments. The early alterations appeared reactive and perhaps reflected ammonia detoxification. Some changes were degenerative and support the view that ammonia exerts a direct toxic effect on astrocytes. It is postulated that these changes may interfere with critical astroglial functions and thereby play a key role in the neurologic dysfunction seen in hyperammonemia.

Original languageEnglish
Pages (from-to)404-414
Number of pages11
JournalJournal of Neuropathology and Experimental Neurology
Volume44
Issue number4
StatePublished - Jan 1 1985

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Ammonia
Astrocytes
Electrons
Hyperammonemia
Smooth Endoplasmic Reticulum
Intermediate Filaments
Rough Endoplasmic Reticulum
Poisons
Neurologic Manifestations
Neuroglia
Mitochondria
Light

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Neuroscience(all)

Cite this

Morphologic effects of ammonia on primary astrocyte cultures. II. Electron microscopic studies. / Gregorios, J. B.; Mozes, L. W.; Norenberg, Michael D.

In: Journal of Neuropathology and Experimental Neurology, Vol. 44, No. 4, 01.01.1985, p. 404-414.

Research output: Contribution to journalArticle

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