Morphologic and metabolic changes in rat osteoblast cultures during the dark reaction with 8-methoxypsoralen

Francis J. Hornicek, George I. Malinin, Kresimir Banovac, Theodore I. Malinin

Research output: Contribution to journalArticle

2 Scopus citations

Abstract

The dark reaction of 8-methoxypsoralen (8-MOP) with cultured rat osteoblasts did not cause significant changes in cellular replication rates or in the synthesis RNA and proteins. Microscopic examination, however, revealed that the dark reaction resulted in massive accumulation of perinuclear lipids and in the statistically significant enhancement of alkaline phosphatase activity. A sharp, and statistically significant, upsurge of lipid synthesis in osteoblasts preceded microscopically detectable accumulation of lipids and occurred only during the initial, but not during the subsequent stages of the dark reaction. These results suggest that in the course of the dark reaction the plasma membrane of osteoblasts is a target of psoralen.

Original languageEnglish (US)
Pages (from-to)185-193
Number of pages9
JournalChemico-Biological Interactions
Volume90
Issue number2
DOIs
StatePublished - Feb 1994

Keywords

  • 8-Methoxypsoralen
  • Dark reaction
  • Lipids
  • Osteoblasts
  • Plasma membrane

ASJC Scopus subject areas

  • Toxicology

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