Morphologic and metabolic changes in rat osteoblast cultures during the dark reaction with 8-methoxypsoralen

Francis J. Hornicek, George I. Malinin, Kresimir Banovac, Theodore Malinin

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

The dark reaction of 8-methoxypsoralen (8-MOP) with cultured rat osteoblasts did not cause significant changes in cellular replication rates or in the synthesis RNA and proteins. Microscopic examination, however, revealed that the dark reaction resulted in massive accumulation of perinuclear lipids and in the statistically significant enhancement of alkaline phosphatase activity. A sharp, and statistically significant, upsurge of lipid synthesis in osteoblasts preceded microscopically detectable accumulation of lipids and occurred only during the initial, but not during the subsequent stages of the dark reaction. These results suggest that in the course of the dark reaction the plasma membrane of osteoblasts is a target of psoralen.

Original languageEnglish
Pages (from-to)185-193
Number of pages9
JournalChemico-Biological Interactions
Volume90
Issue number2
DOIs
StatePublished - Jan 1 1994

Fingerprint

Methoxsalen
Osteoblasts
Cell culture
Rats
Lipids
Ficusin
Cell membranes
Alkaline Phosphatase
Microscopic examination
Cell Membrane
RNA
Proteins

Keywords

  • 8-Methoxypsoralen
  • Dark reaction
  • Lipids
  • Osteoblasts
  • Plasma membrane

ASJC Scopus subject areas

  • Toxicology

Cite this

Morphologic and metabolic changes in rat osteoblast cultures during the dark reaction with 8-methoxypsoralen. / Hornicek, Francis J.; Malinin, George I.; Banovac, Kresimir; Malinin, Theodore.

In: Chemico-Biological Interactions, Vol. 90, No. 2, 01.01.1994, p. 185-193.

Research output: Contribution to journalArticle

Hornicek, Francis J. ; Malinin, George I. ; Banovac, Kresimir ; Malinin, Theodore. / Morphologic and metabolic changes in rat osteoblast cultures during the dark reaction with 8-methoxypsoralen. In: Chemico-Biological Interactions. 1994 ; Vol. 90, No. 2. pp. 185-193.
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