Morphine induces splenocyte trafficking into the CNS

Michael Olin, Seunguk Oh, Sabita Roy, Phillip Peterson, Thomas Molitor

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Opioids significantly alter functional responses of lymphocytes following activation. The opiate Morphine, alters the Th1 to Th2 response and modulates functional responses such as cytolytic activity and T-cell proliferation. Although there has been extensive research involving morphine's effects on lymphocytes, little is known about the effects morphine has on lymphocyte trafficking. The objective of the study was to use in vivo bioluminescent imaging to determine morphine's effect on the trafficking pattern of splenocytes systemically and into the CNS either in a naïve state or following a neuroinflammatory stimulus. A neuroinflammatory response was induced by intracerebrally administering a DNA IFN-γ DNA plasmid into morphinedependent or placebo wildtype mice. Mice with or without a neurostimulus received adoptively transferred firefly luciferase transgenic splenocytes and imaged. Morphine dependence significantly altered the inherent ability of splenocytes to traffic into the spleen, and lead to non-directed chaotic trafficking throughout the animal, including into the CNS. The morphine-mediated effects on trafficking were blocked by the antagonist naltrexone. Morphine dependence intensified splenocyte infiltration into the CNS following neuroinflammation induced by IFN-γ gene transfer. The study precented determined that morphine severely altered the ability of non-activated splenocytes to home to the spleen, inducing extrasplenic trafficking thoughout the animal. In addition to altering the ability of naive splenocyte to traffic to the spleen, this study demonstrated that morphine profoundly exacerbated lymphocyte infiltration into the CNS following a neurostimulus.

Original languageEnglish (US)
Pages (from-to)436-443
Number of pages8
JournalJournal of NeuroImmune Pharmacology
Volume7
Issue number2
DOIs
StatePublished - Jun 1 2012
Externally publishedYes

Fingerprint

Morphine
Aptitude
Morphine Dependence
Spleen
Lymphocytes
Opiate Alkaloids
Firefly Luciferases
Naltrexone
DNA
Lymphocyte Activation
Opioid Analgesics
Plasmids
Placebos
Cell Proliferation
T-Lymphocytes
Research
Genes

Keywords

  • In vivo imaging
  • Lymphocyte trafficking
  • Opiates

ASJC Scopus subject areas

  • Pharmacology
  • Immunology and Allergy
  • Immunology
  • Neuroscience (miscellaneous)
  • Medicine(all)

Cite this

Morphine induces splenocyte trafficking into the CNS. / Olin, Michael; Oh, Seunguk; Roy, Sabita; Peterson, Phillip; Molitor, Thomas.

In: Journal of NeuroImmune Pharmacology, Vol. 7, No. 2, 01.06.2012, p. 436-443.

Research output: Contribution to journalArticle

Olin, Michael ; Oh, Seunguk ; Roy, Sabita ; Peterson, Phillip ; Molitor, Thomas. / Morphine induces splenocyte trafficking into the CNS. In: Journal of NeuroImmune Pharmacology. 2012 ; Vol. 7, No. 2. pp. 436-443.
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