Morphine impairs host innate immune response and increases susceptibility to Streptococcus pneumoniae lung infection

Jinghua Wang, Roderick A. Barke, Richard Charboneau, Sabita Roy

Research output: Contribution to journalArticle

135 Citations (Scopus)

Abstract

Chronic morphine use impairs host innate immune response and increases susceptibility to bacteria and virus. In this study a novel mouse model of chronic morphine treatment, followed by intranasal inoculation with Streptococcus pneumoniae, was used to investigate microbial events and host innate immune response. Our results show that chronic morphine treatment markedly delayed neutrophil recruitment and increased bacterial burden in the lung, spleen, and blood with a subsequent increase in mortality. In morphine-treated animals, before neutrophil recruitment, a significant decrease in TNF-α, IL-1, IL-6, MIP-2, and KC was observed both in bronchoalveolar lavage fluids and in lung tissue. In the early phase of infection, we found that accumulation of galectin-3 in the alveolar space of streptococcus-infected lungs was decreased after morphine treatment. The transcription factor NF-κB in lung resident cells was also inhibited after morphine treatment. Taken together, these results suggest that chronic morphine treatment in an S. pneumoniae infection model suppresses NF-κB gene transcription in lung resident cells, which, in turn, modulates the transcriptional regulation of MIP-2 and inflammatory cytokines. The decreased synthesis of MIP-2 and inflammatory cytokines coupled with the decreased release of galectin-3 result in reduced migration of neutrophils to the site of infection, thereby increasing susceptibility to S. pneumoniae infection after morphine treatment.

Original languageEnglish (US)
Pages (from-to)426-434
Number of pages9
JournalJournal of Immunology
Volume174
Issue number1
DOIs
StatePublished - Jan 1 2005
Externally publishedYes

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Pneumococcal Infections
Innate Immunity
Morphine
Lung
Galectin 3
Neutrophil Infiltration
Therapeutics
Cytokines
Bronchoalveolar Lavage Fluid
Streptococcus pneumoniae
Infection
Streptococcus
Interleukin-1
Interleukin-6
Neutrophils
Transcription Factors
Spleen
Viruses
Bacteria
Mortality

ASJC Scopus subject areas

  • Immunology

Cite this

Morphine impairs host innate immune response and increases susceptibility to Streptococcus pneumoniae lung infection. / Wang, Jinghua; Barke, Roderick A.; Charboneau, Richard; Roy, Sabita.

In: Journal of Immunology, Vol. 174, No. 1, 01.01.2005, p. 426-434.

Research output: Contribution to journalArticle

Wang, Jinghua ; Barke, Roderick A. ; Charboneau, Richard ; Roy, Sabita. / Morphine impairs host innate immune response and increases susceptibility to Streptococcus pneumoniae lung infection. In: Journal of Immunology. 2005 ; Vol. 174, No. 1. pp. 426-434.
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