Monoclonal antibody-defined epitope map of expressed rubella virus protein domains

Jerry S. Wolinsky, Micheline McCarthy, Olive Allen-Cannady, William T. Moore, Rui Jin, Shi Nian Cao, Amy Lovett, Denise Simmons

Research output: Contribution to journalArticle

54 Citations (Scopus)

Abstract

An expanded library of murine monoclonal antibodies (MAbs) was generated by infecting BALB/C mice with the Therien strain of rubella virus (RV) and selecting secreting hybrids by enzyme-linked immunosorbent assay (ELISA) using purified virion targets. A panel of plasmids containing specified RV cDNA fragments was also constructed by using a variety of strategies with pGE374- and pGE374-derived expression vectors. Hybrid RecA-RV-β-galactosidase (LacZ)- or RecA-RV-truncated LacZ-containing proteins collectively representing the entire open reading frame of the structural proteins of RV were overexpressed in Escherichia coli. Bacterial lysates were then probed by ELISA with selected MAbs and by immunoblot following separation by electrophoresis under denaturing conditions. With this approach, MAbs that appeared to react with linear determinants defined epitopes localized within the following domains: MAbs C-1, C-2, and C-8 bind epitopes within the predicted amino-terminal 21 amino acids of the capsid region C9 to C29; MAb C-9 binds to a domain bounded by C64 and C97; MAbs E2-1 through E2-6 bind to the E2 glycoprotein backbone region from E21 to E2115; MAbs E1-18 and E1-20 bind to the El glycoprotein region from E1202 to E1283. MAb E1-18 neutralizes RV infectivity; MAb E1-20 neutralizes infectivity and modestly inhibits hemagglutination. Analyses with selected synthetic peptides have confirmed several of the molecular domains deduced with the expressed proteins. These plasmid constructions and peptides have proven useful in beginning to unravel the molecular organization of several antigenic sites of this human pathogen.

Original languageEnglish
Pages (from-to)3986-3994
Number of pages9
JournalJournal of Virology
Volume65
Issue number8
StatePublished - Aug 1 1991
Externally publishedYes

Fingerprint

Rubella virus
epitopes
Epitopes
monoclonal antibodies
Monoclonal Antibodies
proteins
glycoproteins
Glycoproteins
plasmids
Plasmids
pathogenicity
Enzyme-Linked Immunosorbent Assay
Inbred BALB C Mouse
Galactosidases
enzyme-linked immunosorbent assay
galactosidases
Peptides
Proteins
capsid
synthetic peptides

ASJC Scopus subject areas

  • Immunology

Cite this

Wolinsky, J. S., McCarthy, M., Allen-Cannady, O., Moore, W. T., Jin, R., Cao, S. N., ... Simmons, D. (1991). Monoclonal antibody-defined epitope map of expressed rubella virus protein domains. Journal of Virology, 65(8), 3986-3994.

Monoclonal antibody-defined epitope map of expressed rubella virus protein domains. / Wolinsky, Jerry S.; McCarthy, Micheline; Allen-Cannady, Olive; Moore, William T.; Jin, Rui; Cao, Shi Nian; Lovett, Amy; Simmons, Denise.

In: Journal of Virology, Vol. 65, No. 8, 01.08.1991, p. 3986-3994.

Research output: Contribution to journalArticle

Wolinsky, JS, McCarthy, M, Allen-Cannady, O, Moore, WT, Jin, R, Cao, SN, Lovett, A & Simmons, D 1991, 'Monoclonal antibody-defined epitope map of expressed rubella virus protein domains', Journal of Virology, vol. 65, no. 8, pp. 3986-3994.
Wolinsky JS, McCarthy M, Allen-Cannady O, Moore WT, Jin R, Cao SN et al. Monoclonal antibody-defined epitope map of expressed rubella virus protein domains. Journal of Virology. 1991 Aug 1;65(8):3986-3994.
Wolinsky, Jerry S. ; McCarthy, Micheline ; Allen-Cannady, Olive ; Moore, William T. ; Jin, Rui ; Cao, Shi Nian ; Lovett, Amy ; Simmons, Denise. / Monoclonal antibody-defined epitope map of expressed rubella virus protein domains. In: Journal of Virology. 1991 ; Vol. 65, No. 8. pp. 3986-3994.
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