Monitoring the activity of tyrosinase on a tyramine/dopamine-functionalized surface by force microscopy

Adam B. Braunschweig, Roey Elnathan, Itamar Willner

Research output: Contribution to journalArticlepeer-review

8 Scopus citations

Abstract

Tyrosinase activity is monitored by π-donor-acceptor force interactions between a bipyridinium-modified AFM tip and the biocatalytic reaction product generated on a tyramine- (or dopamine-) modified surface. Upon oxidation of the surface to dopaquinone as a result of tyrosinase activity, force interactions are switched "OFF". After reduction of the resulting surface with ascorbic acid, forces are quantitatively reestablished as a result of the formation of the dopamine-functionalized surfaces. The method provides a general approach to design biosensors using force interactions as the readout signal.

Original languageEnglish (US)
Pages (from-to)2030-2036
Number of pages7
JournalNano Letters
Volume7
Issue number7
DOIs
StatePublished - Jul 1 2007

ASJC Scopus subject areas

  • Bioengineering
  • Chemistry(all)
  • Materials Science(all)
  • Condensed Matter Physics
  • Mechanical Engineering

Fingerprint Dive into the research topics of 'Monitoring the activity of tyrosinase on a tyramine/dopamine-functionalized surface by force microscopy'. Together they form a unique fingerprint.

Cite this