Monitoring steady flow effects on cell distribution in engineered valve tissues by magnetic resonance imaging

Catalina Martinez, Angela Henao, Jose E. Rodriguez, Kyle Padgett, Sharan Ramaswamy

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

In heart valve tissue engineering, assessment of cell migration under dynamic states can provide insights on the evolving tissue structure. We labeled human vascular smooth muscle (SMCs), endothelial (ECs), and bone marrow-derived mesenchymal stem cells (BMSCs) with superparamagnetic iron oxide (SPIO) microparticles and visualized them using magnetic resonance imaging (MRI) under steady flow. We determined that vascular cells were able to remain reasonably viable and proliferate well after being labeled with SPIO microparticles (200 μg/mL) for 48 hours. SPIO-labeled cells were successfully visualized using T2+ contrast. When physiologically representative shear stresses (5-6 dynes/cm2) were applied to SMC-EC coculture-seeded scaffolds, hypointense regions seemed to have decreased after 2 weeks in some locations, whereas others revealed sustained levels of T2+ contrast; similar observations were seen in the case of BMSC-seeded scaffolds. This could be attributable to increased out-of-plane cell migratory activity, which occurred from the fluid-induced mechanical cues received, which was not previously evidenced in static culture. Vascular cells and BMSCs were labeled with remarkably high concentrations of SPIO. Moreover, steady fluid flow enhanced intrascaffold cell migration of vascular SMCs and ECs as well as BMSCs, which, in turn, significantly improved construct cellularity and extracellular collagen content.

Original languageEnglish
JournalMolecular Imaging
Volume12
Issue number7
DOIs
StatePublished - Oct 1 2013
Externally publishedYes

Fingerprint

steady flow
Steady flow
Magnetic resonance
Stem cells
Mesenchymal Stromal Cells
Iron oxides
magnetic resonance
Bone
bone marrow
stem cells
Magnetic Resonance Imaging
Tissue
Muscle
Imaging techniques
smooth muscle
iron oxides
Monitoring
Bone Marrow
cells
Vascular Smooth Muscle

ASJC Scopus subject areas

  • Radiology Nuclear Medicine and imaging
  • Biotechnology
  • Molecular Medicine
  • Biomedical Engineering
  • Condensed Matter Physics

Cite this

Monitoring steady flow effects on cell distribution in engineered valve tissues by magnetic resonance imaging. / Martinez, Catalina; Henao, Angela; Rodriguez, Jose E.; Padgett, Kyle; Ramaswamy, Sharan.

In: Molecular Imaging, Vol. 12, No. 7, 01.10.2013.

Research output: Contribution to journalArticle

Martinez, Catalina ; Henao, Angela ; Rodriguez, Jose E. ; Padgett, Kyle ; Ramaswamy, Sharan. / Monitoring steady flow effects on cell distribution in engineered valve tissues by magnetic resonance imaging. In: Molecular Imaging. 2013 ; Vol. 12, No. 7.
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