Monitoring ligand-receptor interactions by photonic force microscopy.

Sylvia Jeney, Flavio Mor, Roland Koszali, László Forró, Vincent T. Moy

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

We introduce a method for the acquisition of single molecule force measurements of ligand-receptor interactions using the photonic force microscope (PFM). Biotin-functionalized beads, manipulated with an optical trap, and a streptavidin-functionalized coverslip were used to measure the effect of different pulling forces on the lifetime of individual streptavidin-biotin complexes. By optimizing the design of the optical trap and selection of the appropriate bead size, pulling forces in excess of 50 pN were achieved. Based on the amplitude of three-dimensional (3D) thermal position fluctuations of the attached bead, we were able to select for a bead-coverslip interaction that was mediated by a single streptavidin-biotin complex. Moreover, the developed experimental system was greatly accelerated by automation of data acquisition and analysis. In force-dependent kinetic measurements carried out between streptavidin and biotin, we observed that the streptavidin-biotin complex exhibited properties of a catch bond, with the lifetime increasing tenfold when the pulling force increased from 10 to 20 pN. We also show that silica beads were more appropriate than polystyrene beads for the force measurements, as tethers, longer than 200 nm, could be extracted from polystyrene beads.

Original languageEnglish
JournalNanotechnology
Volume21
Issue number25
DOIs
StatePublished - Jun 25 2010
Externally publishedYes

Fingerprint

Photonics
Microscopic examination
Streptavidin
Force measurement
Polystyrenes
Ligands
Biotin
Monitoring
Silicon Dioxide
Data acquisition
Microscopes
Automation
Molecules
Kinetics
Silica
biotin-streptavidin complex
Hot Temperature

ASJC Scopus subject areas

  • Bioengineering
  • Chemistry(all)
  • Electrical and Electronic Engineering
  • Mechanical Engineering
  • Mechanics of Materials
  • Materials Science(all)

Cite this

Monitoring ligand-receptor interactions by photonic force microscopy. / Jeney, Sylvia; Mor, Flavio; Koszali, Roland; Forró, László; Moy, Vincent T.

In: Nanotechnology, Vol. 21, No. 25, 25.06.2010.

Research output: Contribution to journalArticle

Jeney, Sylvia ; Mor, Flavio ; Koszali, Roland ; Forró, László ; Moy, Vincent T. / Monitoring ligand-receptor interactions by photonic force microscopy. In: Nanotechnology. 2010 ; Vol. 21, No. 25.
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