Abstract
Parietovisceral ganglia from Aplysia californica were incubated in medium containing leucine-8H. Single, identified nerve cell somas were isolated from the ganglia, and their proteins extracted and separated by electrophoresis on 5 % SDS-polyacrylamide gels. The distribution of total or newly synthesized proteins from the single neurons was determined by staining or slicing and liquid scintillation counting of the gels. Experiments showed that: (a) a number of proteins were being synthesized in abundance in the nerve cells; (b) different, identified neurons showed reproducibly different labeling patterns in the gels; (c) cells R2 and R15, which showed different distributions of radioactivity in the gels, had similar staining patterns; and (d) there was significant incorporation into material of high (> 75,000) molecular weight in most of the cells.
Original language | English (US) |
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Pages (from-to) | 26-40 |
Number of pages | 15 |
Journal | Journal of General Physiology |
Volume | 57 |
Issue number | 1 |
DOIs | |
State | Published - Jan 1 1971 |
ASJC Scopus subject areas
- Physiology