Molecular weight dependence of calcification of polyethylene glycol hydrogels

S. F A Hossainy, J. A. Hubbell

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

In vivo calcification of polyethylene glycol diacrylate (PEG DA) hydrogels of molecular weight (MW) 400,1000, 4000, 6000 and 10000 and polyethylene glycol tetraacrylate (PEG TA) of MW 18500 was investigated using a rat subcutaneous model. This study was performed in 4-wk-old rats for durations of 1, 3, 6 and 8 wk. The results indicate a strong dependence of calcification upon the MW of the PEG precursor or the MW between crosslinks. Results for gels implanted for 6 wk show that calcification was maximal at a PEG MW of 1000 (224 mg/g ± 12.8, n = 4) (mean ± SEM) with less at MW = 400 (23.0 mg/g ± 9.30, n = 4) and considerably less at higher MWs, e.g. for MW = 10000 (0.23 mg/g ± 0.01, n = 4). Results for other time periods indicate a similar calcification trend. The extent of calcification of gels from PEG TA (MW = 18500) was intermediate (1.09 mg/g ± 0.43, n = 3) between PEG DA (MW = 6000) (1.39 mg/g ± 0.42, n = 6) and PEG DA (MW = 10000) at 6 wk, i.e. calcification depended upon the PEG MW between crosslinks. When composite gels were implanted, such that a highly calcifying gel (MW = 400 or 1000) was encapsulated within a gel of low calcification (MW = 4000), the gel inside calcified to at least the same extent as if it had not been encapsulated. Thus, direct contact with tissues is apparently not necessary for calcification to occur. Energy dispersive X-ray spectroscopy was performed on the mineral deposits in the gels and a P:Ca ratio of 0.67 ± 0.04 (95% confidence interval) for MW 1000 gels and 0.60 ± 0.07 for MW 400 gels was found to be consistent with deposition of Ca3(PO4)2.

Original languageEnglish
Pages (from-to)921-925
Number of pages5
JournalBiomaterials
Volume15
Issue number11
DOIs
StatePublished - Sep 1 1994
Externally publishedYes

Fingerprint

Hydrogels
Polyethylene glycols
Molecular Weight
Molecular weight
Gels
Rats
X-Ray Emission Spectrometry
Mineral resources

Keywords

  • Calcification
  • Hydrogels
  • Molecular weight dependence
  • Polyethylene glycols

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Biomedical Engineering

Cite this

Molecular weight dependence of calcification of polyethylene glycol hydrogels. / Hossainy, S. F A; Hubbell, J. A.

In: Biomaterials, Vol. 15, No. 11, 01.09.1994, p. 921-925.

Research output: Contribution to journalArticle

Hossainy, S. F A ; Hubbell, J. A. / Molecular weight dependence of calcification of polyethylene glycol hydrogels. In: Biomaterials. 1994 ; Vol. 15, No. 11. pp. 921-925.
@article{a70ac4aa2c3d4404b3bf1d7b9cc4ae27,
title = "Molecular weight dependence of calcification of polyethylene glycol hydrogels",
abstract = "In vivo calcification of polyethylene glycol diacrylate (PEG DA) hydrogels of molecular weight (MW) 400,1000, 4000, 6000 and 10000 and polyethylene glycol tetraacrylate (PEG TA) of MW 18500 was investigated using a rat subcutaneous model. This study was performed in 4-wk-old rats for durations of 1, 3, 6 and 8 wk. The results indicate a strong dependence of calcification upon the MW of the PEG precursor or the MW between crosslinks. Results for gels implanted for 6 wk show that calcification was maximal at a PEG MW of 1000 (224 mg/g ± 12.8, n = 4) (mean ± SEM) with less at MW = 400 (23.0 mg/g ± 9.30, n = 4) and considerably less at higher MWs, e.g. for MW = 10000 (0.23 mg/g ± 0.01, n = 4). Results for other time periods indicate a similar calcification trend. The extent of calcification of gels from PEG TA (MW = 18500) was intermediate (1.09 mg/g ± 0.43, n = 3) between PEG DA (MW = 6000) (1.39 mg/g ± 0.42, n = 6) and PEG DA (MW = 10000) at 6 wk, i.e. calcification depended upon the PEG MW between crosslinks. When composite gels were implanted, such that a highly calcifying gel (MW = 400 or 1000) was encapsulated within a gel of low calcification (MW = 4000), the gel inside calcified to at least the same extent as if it had not been encapsulated. Thus, direct contact with tissues is apparently not necessary for calcification to occur. Energy dispersive X-ray spectroscopy was performed on the mineral deposits in the gels and a P:Ca ratio of 0.67 ± 0.04 (95{\%} confidence interval) for MW 1000 gels and 0.60 ± 0.07 for MW 400 gels was found to be consistent with deposition of Ca3(PO4)2.",
keywords = "Calcification, Hydrogels, Molecular weight dependence, Polyethylene glycols",
author = "Hossainy, {S. F A} and Hubbell, {J. A.}",
year = "1994",
month = "9",
day = "1",
doi = "10.1016/0142-9612(94)90118-X",
language = "English",
volume = "15",
pages = "921--925",
journal = "Biomaterials",
issn = "0142-9612",
publisher = "Elsevier BV",
number = "11",

}

TY - JOUR

T1 - Molecular weight dependence of calcification of polyethylene glycol hydrogels

AU - Hossainy, S. F A

AU - Hubbell, J. A.

PY - 1994/9/1

Y1 - 1994/9/1

N2 - In vivo calcification of polyethylene glycol diacrylate (PEG DA) hydrogels of molecular weight (MW) 400,1000, 4000, 6000 and 10000 and polyethylene glycol tetraacrylate (PEG TA) of MW 18500 was investigated using a rat subcutaneous model. This study was performed in 4-wk-old rats for durations of 1, 3, 6 and 8 wk. The results indicate a strong dependence of calcification upon the MW of the PEG precursor or the MW between crosslinks. Results for gels implanted for 6 wk show that calcification was maximal at a PEG MW of 1000 (224 mg/g ± 12.8, n = 4) (mean ± SEM) with less at MW = 400 (23.0 mg/g ± 9.30, n = 4) and considerably less at higher MWs, e.g. for MW = 10000 (0.23 mg/g ± 0.01, n = 4). Results for other time periods indicate a similar calcification trend. The extent of calcification of gels from PEG TA (MW = 18500) was intermediate (1.09 mg/g ± 0.43, n = 3) between PEG DA (MW = 6000) (1.39 mg/g ± 0.42, n = 6) and PEG DA (MW = 10000) at 6 wk, i.e. calcification depended upon the PEG MW between crosslinks. When composite gels were implanted, such that a highly calcifying gel (MW = 400 or 1000) was encapsulated within a gel of low calcification (MW = 4000), the gel inside calcified to at least the same extent as if it had not been encapsulated. Thus, direct contact with tissues is apparently not necessary for calcification to occur. Energy dispersive X-ray spectroscopy was performed on the mineral deposits in the gels and a P:Ca ratio of 0.67 ± 0.04 (95% confidence interval) for MW 1000 gels and 0.60 ± 0.07 for MW 400 gels was found to be consistent with deposition of Ca3(PO4)2.

AB - In vivo calcification of polyethylene glycol diacrylate (PEG DA) hydrogels of molecular weight (MW) 400,1000, 4000, 6000 and 10000 and polyethylene glycol tetraacrylate (PEG TA) of MW 18500 was investigated using a rat subcutaneous model. This study was performed in 4-wk-old rats for durations of 1, 3, 6 and 8 wk. The results indicate a strong dependence of calcification upon the MW of the PEG precursor or the MW between crosslinks. Results for gels implanted for 6 wk show that calcification was maximal at a PEG MW of 1000 (224 mg/g ± 12.8, n = 4) (mean ± SEM) with less at MW = 400 (23.0 mg/g ± 9.30, n = 4) and considerably less at higher MWs, e.g. for MW = 10000 (0.23 mg/g ± 0.01, n = 4). Results for other time periods indicate a similar calcification trend. The extent of calcification of gels from PEG TA (MW = 18500) was intermediate (1.09 mg/g ± 0.43, n = 3) between PEG DA (MW = 6000) (1.39 mg/g ± 0.42, n = 6) and PEG DA (MW = 10000) at 6 wk, i.e. calcification depended upon the PEG MW between crosslinks. When composite gels were implanted, such that a highly calcifying gel (MW = 400 or 1000) was encapsulated within a gel of low calcification (MW = 4000), the gel inside calcified to at least the same extent as if it had not been encapsulated. Thus, direct contact with tissues is apparently not necessary for calcification to occur. Energy dispersive X-ray spectroscopy was performed on the mineral deposits in the gels and a P:Ca ratio of 0.67 ± 0.04 (95% confidence interval) for MW 1000 gels and 0.60 ± 0.07 for MW 400 gels was found to be consistent with deposition of Ca3(PO4)2.

KW - Calcification

KW - Hydrogels

KW - Molecular weight dependence

KW - Polyethylene glycols

UR - http://www.scopus.com/inward/record.url?scp=0028500680&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0028500680&partnerID=8YFLogxK

U2 - 10.1016/0142-9612(94)90118-X

DO - 10.1016/0142-9612(94)90118-X

M3 - Article

VL - 15

SP - 921

EP - 925

JO - Biomaterials

JF - Biomaterials

SN - 0142-9612

IS - 11

ER -