TY - JOUR
T1 - Molecular typing of major histocompatibility complex class I alleles in the Indian rhesus macaque which restrict SIV CD8+ T cell epitopes
AU - Kaizu, Masahiko
AU - Borchardt, Gretta J.
AU - Glidden, Chrystal E.
AU - Fisk, Debra L.
AU - Loffredo, John T.
AU - Watkins, David I.
AU - Rehrauer, William M.
N1 - Funding Information:
Acknowledgments We express our gratitude to Jess Maxwell, Tim Jacoby, Julie Leblanc, Brock Christiansen, Millicent Schultz, Elizabeth Meek, and Christopher Murvine as previous members of the laboratory for their extensive assistance to the MHC class I PCR-SSP typing project and gratefully recognize Rudy Rudersdorf and Lyle Wallace for their significant contributions of transfectant cell lines and MHC class I cDNA derived sequences. We acknowledge Nancy A. Wilson, David O’Connor, Thomas C. Friedrich, Adrian McDermott, and Roger Wiseman for their insightful discussions. We also collectively thank the Immunology, Virology, and DNA Sequencing Core Laboratories at the National Primate Research Center, University of Wisconsin–Madison for their technical assistance. We appreciate Amanda Espinosa for her help and administrative contributions to our laboratory. Finally, we value the opportunity to serve as a MHC typing service for numerous investigators, without their interest and support this work would not have been possible. This research was supported by National Institutes of Health (NIH) grant R24 RR016038 and NIAID contract no. HHSN266200400088C. This publication was also made possible in part by Grant Number P51 RR000167 from the National Center for Research Resources (NCRR), a component of the National Institutes of Health (NIH), to the Wisconsin National Primate Research Center, University of Wisconsin–Madison. This research was conducted at a facility constructed with support from Research Facilities Improvement Program grant numbers RR15459-01 and RR020141-01. This publication’s contents are solely the responsibility of the authors and do not necessarily represent the official views of NCRR or NIH.
PY - 2007/9
Y1 - 2007/9
N2 - The utility of the rhesus macaque as an animal model in both HIV vaccine development and pathogenesis studies necessitates the development of accurate and efficient major histocompatibility complex (MHC) genotyping technologies. In this paper, we describe the development and application of allele-specific polymerase chain reaction (PCR) amplification for the simultaneous detection of eight MHC class I alleles from the rhesus macaque (Macaca mulatta) of Indian descent. These alleles were selected, as they have been implicated in the restriction of CD8+ T cell epitopes of simian immunodeficiency virus (SIV). Molecular typing of Mamu-A*01, Mamu-A*02, Mamu-A*08, Mamu-A*11, Mamu-B*01, Mamu-B*03, Mamu-B*04, and Mamu-B*17 was conducted in a high throughput fashion using genomic DNA. Our amplification strategy included a conserved internal control target to minimize false negative results and can be completed in less than 5 h. We have genotyped over 4,000 animals to establish allele frequencies from colonies all over the western hemisphere. The ability to identify MHC-defined rhesus macaques will greatly enhance investigation of the immune responses, which are responsible for the control of viral replication. Furthermore, application of this technically simple and accurate typing method should facilitate selection, utilization, and breeding of rhesus macaques for AIDS virus pathogenesis and vaccine studies.
AB - The utility of the rhesus macaque as an animal model in both HIV vaccine development and pathogenesis studies necessitates the development of accurate and efficient major histocompatibility complex (MHC) genotyping technologies. In this paper, we describe the development and application of allele-specific polymerase chain reaction (PCR) amplification for the simultaneous detection of eight MHC class I alleles from the rhesus macaque (Macaca mulatta) of Indian descent. These alleles were selected, as they have been implicated in the restriction of CD8+ T cell epitopes of simian immunodeficiency virus (SIV). Molecular typing of Mamu-A*01, Mamu-A*02, Mamu-A*08, Mamu-A*11, Mamu-B*01, Mamu-B*03, Mamu-B*04, and Mamu-B*17 was conducted in a high throughput fashion using genomic DNA. Our amplification strategy included a conserved internal control target to minimize false negative results and can be completed in less than 5 h. We have genotyped over 4,000 animals to establish allele frequencies from colonies all over the western hemisphere. The ability to identify MHC-defined rhesus macaques will greatly enhance investigation of the immune responses, which are responsible for the control of viral replication. Furthermore, application of this technically simple and accurate typing method should facilitate selection, utilization, and breeding of rhesus macaques for AIDS virus pathogenesis and vaccine studies.
KW - Genotyping
KW - MHC
KW - Rhesus macaque
KW - SIV
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U2 - 10.1007/s00251-007-0233-7
DO - 10.1007/s00251-007-0233-7
M3 - Article
C2 - 17641886
AN - SCOPUS:34548685405
VL - 59
SP - 693
EP - 703
JO - Immunogenetics
JF - Immunogenetics
SN - 0093-7711
IS - 9
ER -