Molecular structure and functional testing of human L1CAM: An interspecies comparison

Mary Louise Hlavin, Vance Lemmon

Research output: Contribution to journalArticle

118 Citations (Scopus)

Abstract

The rodent, avian, and insect L1-like cell adhesion molecules are members of the immunoglobulin superfamily that have been implicated in axon growth. We have isolated an L1-like molecule from human brain and found that it also supports neurite growth in vitro. We have also cloned and sequenced the entire coding region of human L1CAM and found that it shows a very high degree of homology to mouse L1cam, with 92% identity at the amino acid level. This similarity suggests that L1CAM is an important molecule in normal human nervous system development and nerve regeneration. Overall, there is substantially less homology to chick Ng-CAM; they are 40% identical at the amino acid level but many regions are highly conserved. Comparison of the sequences from human, mouse, chick, and Drosophila indicates that the L1 immunoglobulin domain 2 and fibronectin type III domain 2 are strongly conserved and thus are likely functionally important.

Original languageEnglish
Pages (from-to)416-423
Number of pages8
JournalGenomics
Volume11
Issue number2
DOIs
StatePublished - Jan 1 1991
Externally publishedYes

Fingerprint

Neural Cell Adhesion Molecule L1
Molecular Structure
Neuron-Glia Cell Adhesion Molecules
Amino Acids
Nerve Regeneration
Neurites
Growth
Nervous System
Drosophila
Axons
Insects
Immunoglobulins
Rodentia
Brain

ASJC Scopus subject areas

  • Genetics

Cite this

Molecular structure and functional testing of human L1CAM : An interspecies comparison. / Hlavin, Mary Louise; Lemmon, Vance.

In: Genomics, Vol. 11, No. 2, 01.01.1991, p. 416-423.

Research output: Contribution to journalArticle

@article{88dd339b27844f19baca5695ea65354f,
title = "Molecular structure and functional testing of human L1CAM: An interspecies comparison",
abstract = "The rodent, avian, and insect L1-like cell adhesion molecules are members of the immunoglobulin superfamily that have been implicated in axon growth. We have isolated an L1-like molecule from human brain and found that it also supports neurite growth in vitro. We have also cloned and sequenced the entire coding region of human L1CAM and found that it shows a very high degree of homology to mouse L1cam, with 92{\%} identity at the amino acid level. This similarity suggests that L1CAM is an important molecule in normal human nervous system development and nerve regeneration. Overall, there is substantially less homology to chick Ng-CAM; they are 40{\%} identical at the amino acid level but many regions are highly conserved. Comparison of the sequences from human, mouse, chick, and Drosophila indicates that the L1 immunoglobulin domain 2 and fibronectin type III domain 2 are strongly conserved and thus are likely functionally important.",
author = "Hlavin, {Mary Louise} and Vance Lemmon",
year = "1991",
month = "1",
day = "1",
doi = "10.1016/0888-7543(91)90150-D",
language = "English",
volume = "11",
pages = "416--423",
journal = "Genomics",
issn = "0888-7543",
publisher = "Academic Press Inc.",
number = "2",

}

TY - JOUR

T1 - Molecular structure and functional testing of human L1CAM

T2 - An interspecies comparison

AU - Hlavin, Mary Louise

AU - Lemmon, Vance

PY - 1991/1/1

Y1 - 1991/1/1

N2 - The rodent, avian, and insect L1-like cell adhesion molecules are members of the immunoglobulin superfamily that have been implicated in axon growth. We have isolated an L1-like molecule from human brain and found that it also supports neurite growth in vitro. We have also cloned and sequenced the entire coding region of human L1CAM and found that it shows a very high degree of homology to mouse L1cam, with 92% identity at the amino acid level. This similarity suggests that L1CAM is an important molecule in normal human nervous system development and nerve regeneration. Overall, there is substantially less homology to chick Ng-CAM; they are 40% identical at the amino acid level but many regions are highly conserved. Comparison of the sequences from human, mouse, chick, and Drosophila indicates that the L1 immunoglobulin domain 2 and fibronectin type III domain 2 are strongly conserved and thus are likely functionally important.

AB - The rodent, avian, and insect L1-like cell adhesion molecules are members of the immunoglobulin superfamily that have been implicated in axon growth. We have isolated an L1-like molecule from human brain and found that it also supports neurite growth in vitro. We have also cloned and sequenced the entire coding region of human L1CAM and found that it shows a very high degree of homology to mouse L1cam, with 92% identity at the amino acid level. This similarity suggests that L1CAM is an important molecule in normal human nervous system development and nerve regeneration. Overall, there is substantially less homology to chick Ng-CAM; they are 40% identical at the amino acid level but many regions are highly conserved. Comparison of the sequences from human, mouse, chick, and Drosophila indicates that the L1 immunoglobulin domain 2 and fibronectin type III domain 2 are strongly conserved and thus are likely functionally important.

UR - http://www.scopus.com/inward/record.url?scp=0026010655&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0026010655&partnerID=8YFLogxK

U2 - 10.1016/0888-7543(91)90150-D

DO - 10.1016/0888-7543(91)90150-D

M3 - Article

C2 - 1769655

AN - SCOPUS:0026010655

VL - 11

SP - 416

EP - 423

JO - Genomics

JF - Genomics

SN - 0888-7543

IS - 2

ER -