Molecular reorganization of lipid bilayers by complement

A possible mechanism for membranolysis

A. F. Esser, W. P. Kolb, E. R. Podack, H. J. Muller-Eberhard

Research output: Contribution to journalArticle

58 Citations (Scopus)

Abstract

The interaction between the membrane attack complex (MAC) of complement and flat lipid bilayers was investigated. Using spin-labeled derivates of phospholipids and cholesterol and electron paramagnetic resonance spectroscopy, we measured the penetration of the MAC into bilayers and its influence on the order of bilayers. The MAC precursor components C5b-6, C7, C8, and C9 did not exert any measurable influence on lipid membranes. Functional C5b-7 was shown to interact strongly with the bilayer surface without deep penetration into the bilayer. Formation of C5b-8 and especially C5b-9 caused a marked change in the anisotropy of spectra from probes located within the hydrocarbon phase. The spectral changes are not caused by changes in probe rotation and, in the case of cholesterol probes, are not due to direct probe-protein interactions. For these reasons we interpret the spectral changes to be the result of reorientation of ordered bilayer lipids effected by strong binding of phospholipids to MAC proteins.

Original languageEnglish
Pages (from-to)1410-1414
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume76
Issue number3
StatePublished - Jan 1 1979
Externally publishedYes

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Complement Membrane Attack Complex
Lipid Bilayers
Phospholipids
Cholesterol
Anisotropy
Electron Spin Resonance Spectroscopy
Membrane Lipids
Hydrocarbons
Spectrum Analysis
Proteins

ASJC Scopus subject areas

  • General
  • Genetics

Cite this

Molecular reorganization of lipid bilayers by complement : A possible mechanism for membranolysis. / Esser, A. F.; Kolb, W. P.; Podack, E. R.; Muller-Eberhard, H. J.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 76, No. 3, 01.01.1979, p. 1410-1414.

Research output: Contribution to journalArticle

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