TY - JOUR
T1 - Molecular recognition of concanavalin A on mannoside diacetylene lipid monolayer at the air-water interface
AU - Wang, Shaopeng
AU - Leblanc, Roger M.
PY - 1999/7/15
Y1 - 1999/7/15
N2 - The interaction of p-10,12-pentacosadiyne-1-n-phenylamide α-D-mannopyranoside (MPDA) with protein concanavalin A (Con A) was studied at the air/water interface. The expansion of molecular area of PDA (10,12-pentacosadiynoic acid)/MPDA mixed monolayer after injection of Con A in subphase shows strong interaction between Con A and the monolayer. The maximum expansion of molecular area decreases as the molar ratio of MPDA increases due to the steric hindrance effect. By using enzyme mannosidase to cut-off the mannoside headgroup of MPDA, expansion of molecular area was greatly reduced, indicating that the binding of Con A is specific to the mannoside headgroup. The kinetics of the binding fits to the first order bimolecular reaction model. Fluorescence quenching of fluorescein isothiocyanate labeled Con A after injection into the subphase gives a direct proof of the molecular recognition. Copyright (C) 1999 Elsevier Science B.V.
AB - The interaction of p-10,12-pentacosadiyne-1-n-phenylamide α-D-mannopyranoside (MPDA) with protein concanavalin A (Con A) was studied at the air/water interface. The expansion of molecular area of PDA (10,12-pentacosadiynoic acid)/MPDA mixed monolayer after injection of Con A in subphase shows strong interaction between Con A and the monolayer. The maximum expansion of molecular area decreases as the molar ratio of MPDA increases due to the steric hindrance effect. By using enzyme mannosidase to cut-off the mannoside headgroup of MPDA, expansion of molecular area was greatly reduced, indicating that the binding of Con A is specific to the mannoside headgroup. The kinetics of the binding fits to the first order bimolecular reaction model. Fluorescence quenching of fluorescein isothiocyanate labeled Con A after injection into the subphase gives a direct proof of the molecular recognition. Copyright (C) 1999 Elsevier Science B.V.
KW - Concanavalin A
KW - Langmuir monolayer
KW - Mannoside diacetylene lipid
KW - Molecular recognition
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U2 - 10.1016/S0005-2736(99)00079-6
DO - 10.1016/S0005-2736(99)00079-6
M3 - Article
C2 - 10407081
AN - SCOPUS:0033038419
VL - 1419
SP - 307
EP - 312
JO - Biochimica et Biophysica Acta - Biomembranes
JF - Biochimica et Biophysica Acta - Biomembranes
SN - 0005-2736
IS - 2
ER -