Molecular recognition of concanavalin A on mannoside diacetylene lipid monolayer at the air-water interface

Shaopeng Wang, Roger M. Leblanc

Research output: Contribution to journalArticlepeer-review

10 Scopus citations

Abstract

The interaction of p-10,12-pentacosadiyne-1-n-phenylamide α-D-mannopyranoside (MPDA) with protein concanavalin A (Con A) was studied at the air/water interface. The expansion of molecular area of PDA (10,12-pentacosadiynoic acid)/MPDA mixed monolayer after injection of Con A in subphase shows strong interaction between Con A and the monolayer. The maximum expansion of molecular area decreases as the molar ratio of MPDA increases due to the steric hindrance effect. By using enzyme mannosidase to cut-off the mannoside headgroup of MPDA, expansion of molecular area was greatly reduced, indicating that the binding of Con A is specific to the mannoside headgroup. The kinetics of the binding fits to the first order bimolecular reaction model. Fluorescence quenching of fluorescein isothiocyanate labeled Con A after injection into the subphase gives a direct proof of the molecular recognition. Copyright (C) 1999 Elsevier Science B.V.

Original languageEnglish (US)
Pages (from-to)307-312
Number of pages6
JournalBiochimica et Biophysica Acta - Biomembranes
Volume1419
Issue number2
DOIs
StatePublished - Jul 15 1999

Keywords

  • Concanavalin A
  • Langmuir monolayer
  • Mannoside diacetylene lipid
  • Molecular recognition

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Biophysics

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