Molecular receptive range variation among mouse odorant receptors for aliphatic carboxylic acids

Sarah E. Repicky, Charles W Luetje

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

The ability of mammals to identify and distinguish among many thousands of different odorants suggests a combinatorial use of odorant receptors, with each receptor detecting multiple odorants and each odorant interacting with multiple receptors. Numerous receptors may be devoted to the sampling of particularly important regions of odor space. In this study, we explore the similarities and differences in the molecular receptive ranges of four mouse odorant receptors (MOR23-1, MOR31-4, MOR32-11 and MOR40-4), which have previously been identified as receptors for aliphatic carboxylic acids. Each receptor was expressed in Xenopus oocytes, along with Gαolf and the cystic fibrosis transmembrane regulator to allow electrophysiological assay of receptor responses. We find that even though these receptors are relatively unrelated, there is extensive overlap among their receptive ranges. That is, these receptors sample a similar region of odor space. However, the receptive range of each receptor is unique. Thus, these receptors contribute to the depth of coverage of this small region of odor space. Such a group of receptors with overlapping, but distinct receptive ranges, may participate in making fine distinctions among complex mixtures of closely related odorant compounds.

Original languageEnglish
Pages (from-to)193-202
Number of pages10
JournalJournal of Neurochemistry
Volume109
Issue number1
DOIs
StatePublished - Apr 1 2009

Fingerprint

Odorant Receptors
Odors
Carboxylic Acids
Fatty Acids
Mammals
Complex Mixtures
Assays
Sampling
Golf
Xenopus
Odorants
Cystic Fibrosis
Oocytes

Keywords

  • Electrophysiology
  • Olfactory receptors
  • Xenopus oocytes

ASJC Scopus subject areas

  • Biochemistry
  • Cellular and Molecular Neuroscience

Cite this

Molecular receptive range variation among mouse odorant receptors for aliphatic carboxylic acids. / Repicky, Sarah E.; Luetje, Charles W.

In: Journal of Neurochemistry, Vol. 109, No. 1, 01.04.2009, p. 193-202.

Research output: Contribution to journalArticle

Repicky, Sarah E. ; Luetje, Charles W. / Molecular receptive range variation among mouse odorant receptors for aliphatic carboxylic acids. In: Journal of Neurochemistry. 2009 ; Vol. 109, No. 1. pp. 193-202.
@article{11bdfaec9dc04f6e8085774d008688c6,
title = "Molecular receptive range variation among mouse odorant receptors for aliphatic carboxylic acids",
abstract = "The ability of mammals to identify and distinguish among many thousands of different odorants suggests a combinatorial use of odorant receptors, with each receptor detecting multiple odorants and each odorant interacting with multiple receptors. Numerous receptors may be devoted to the sampling of particularly important regions of odor space. In this study, we explore the similarities and differences in the molecular receptive ranges of four mouse odorant receptors (MOR23-1, MOR31-4, MOR32-11 and MOR40-4), which have previously been identified as receptors for aliphatic carboxylic acids. Each receptor was expressed in Xenopus oocytes, along with Gαolf and the cystic fibrosis transmembrane regulator to allow electrophysiological assay of receptor responses. We find that even though these receptors are relatively unrelated, there is extensive overlap among their receptive ranges. That is, these receptors sample a similar region of odor space. However, the receptive range of each receptor is unique. Thus, these receptors contribute to the depth of coverage of this small region of odor space. Such a group of receptors with overlapping, but distinct receptive ranges, may participate in making fine distinctions among complex mixtures of closely related odorant compounds.",
keywords = "Electrophysiology, Olfactory receptors, Xenopus oocytes",
author = "Repicky, {Sarah E.} and Luetje, {Charles W}",
year = "2009",
month = "4",
day = "1",
doi = "10.1111/j.1471-4159.2009.05925.x",
language = "English",
volume = "109",
pages = "193--202",
journal = "Journal of Neurochemistry",
issn = "0022-3042",
publisher = "Wiley-Blackwell",
number = "1",

}

TY - JOUR

T1 - Molecular receptive range variation among mouse odorant receptors for aliphatic carboxylic acids

AU - Repicky, Sarah E.

AU - Luetje, Charles W

PY - 2009/4/1

Y1 - 2009/4/1

N2 - The ability of mammals to identify and distinguish among many thousands of different odorants suggests a combinatorial use of odorant receptors, with each receptor detecting multiple odorants and each odorant interacting with multiple receptors. Numerous receptors may be devoted to the sampling of particularly important regions of odor space. In this study, we explore the similarities and differences in the molecular receptive ranges of four mouse odorant receptors (MOR23-1, MOR31-4, MOR32-11 and MOR40-4), which have previously been identified as receptors for aliphatic carboxylic acids. Each receptor was expressed in Xenopus oocytes, along with Gαolf and the cystic fibrosis transmembrane regulator to allow electrophysiological assay of receptor responses. We find that even though these receptors are relatively unrelated, there is extensive overlap among their receptive ranges. That is, these receptors sample a similar region of odor space. However, the receptive range of each receptor is unique. Thus, these receptors contribute to the depth of coverage of this small region of odor space. Such a group of receptors with overlapping, but distinct receptive ranges, may participate in making fine distinctions among complex mixtures of closely related odorant compounds.

AB - The ability of mammals to identify and distinguish among many thousands of different odorants suggests a combinatorial use of odorant receptors, with each receptor detecting multiple odorants and each odorant interacting with multiple receptors. Numerous receptors may be devoted to the sampling of particularly important regions of odor space. In this study, we explore the similarities and differences in the molecular receptive ranges of four mouse odorant receptors (MOR23-1, MOR31-4, MOR32-11 and MOR40-4), which have previously been identified as receptors for aliphatic carboxylic acids. Each receptor was expressed in Xenopus oocytes, along with Gαolf and the cystic fibrosis transmembrane regulator to allow electrophysiological assay of receptor responses. We find that even though these receptors are relatively unrelated, there is extensive overlap among their receptive ranges. That is, these receptors sample a similar region of odor space. However, the receptive range of each receptor is unique. Thus, these receptors contribute to the depth of coverage of this small region of odor space. Such a group of receptors with overlapping, but distinct receptive ranges, may participate in making fine distinctions among complex mixtures of closely related odorant compounds.

KW - Electrophysiology

KW - Olfactory receptors

KW - Xenopus oocytes

UR - http://www.scopus.com/inward/record.url?scp=61849133441&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=61849133441&partnerID=8YFLogxK

U2 - 10.1111/j.1471-4159.2009.05925.x

DO - 10.1111/j.1471-4159.2009.05925.x

M3 - Article

VL - 109

SP - 193

EP - 202

JO - Journal of Neurochemistry

JF - Journal of Neurochemistry

SN - 0022-3042

IS - 1

ER -