Abstract
The localization of MHC-encoded determinants recognized by hapten- and allo-specific cytotoxic T cells was analyzed with the use of cell lines expressing recombinant H-2D(d) and H-2L(d) MHC products. Bulk cultures of CTL against TNP-self, FITC-self, and AED-self recognized self determinants associated with the N/C1 domains of both D(d) and L(d) products. A number of allo- and hapten-specific CTL clones were also tested for recognition of the recombinant MHC products. The allo clones specific for L(d) or D(d) antigens recognized the respective N/C1-associated determinants. In addition, all clones generated against H-2(q) and known to cross-react with H-2D(d) antigens recognized determinants associated with the N/C1-associated D(d) determinants. Thus, some of the results obtained with CTL parallel, whereas others contrast with, those findings obtained with monoclonal anti-H-2 antibodies. Similar to the observations made with the monoclonal antibodies, no determinant as defined by T cells has been found to be lost as a result of the interaction between the N/C1 and C2/M domains of the L(d) and D(d) proteins. Nor did our studies detect the presence of new antigens resulting from the interaction of these gene products. However, the present T cell findings continue to contrast previous results demonstrating that antibody interaction with class I products includes recognition of C2/M-associated epitopes.
| Original language | English |
|---|---|
| Pages (from-to) | 677-683 |
| Number of pages | 7 |
| Journal | Journal of Immunology |
| Volume | 134 |
| Issue number | 2 |
| State | Published - Jan 1 1985 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Immunology
Cite this
Molecular localization of allogeneic and self determinants recognized by bulk and clonal populations of cytotoxic T cells. / Levy, Robert B; Ozato, K.; Richardson, J. C.; Bluestone, J. A.
In: Journal of Immunology, Vol. 134, No. 2, 01.01.1985, p. 677-683.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Molecular localization of allogeneic and self determinants recognized by bulk and clonal populations of cytotoxic T cells
AU - Levy, Robert B
AU - Ozato, K.
AU - Richardson, J. C.
AU - Bluestone, J. A.
PY - 1985/1/1
Y1 - 1985/1/1
N2 - The localization of MHC-encoded determinants recognized by hapten- and allo-specific cytotoxic T cells was analyzed with the use of cell lines expressing recombinant H-2D(d) and H-2L(d) MHC products. Bulk cultures of CTL against TNP-self, FITC-self, and AED-self recognized self determinants associated with the N/C1 domains of both D(d) and L(d) products. A number of allo- and hapten-specific CTL clones were also tested for recognition of the recombinant MHC products. The allo clones specific for L(d) or D(d) antigens recognized the respective N/C1-associated determinants. In addition, all clones generated against H-2(q) and known to cross-react with H-2D(d) antigens recognized determinants associated with the N/C1-associated D(d) determinants. Thus, some of the results obtained with CTL parallel, whereas others contrast with, those findings obtained with monoclonal anti-H-2 antibodies. Similar to the observations made with the monoclonal antibodies, no determinant as defined by T cells has been found to be lost as a result of the interaction between the N/C1 and C2/M domains of the L(d) and D(d) proteins. Nor did our studies detect the presence of new antigens resulting from the interaction of these gene products. However, the present T cell findings continue to contrast previous results demonstrating that antibody interaction with class I products includes recognition of C2/M-associated epitopes.
AB - The localization of MHC-encoded determinants recognized by hapten- and allo-specific cytotoxic T cells was analyzed with the use of cell lines expressing recombinant H-2D(d) and H-2L(d) MHC products. Bulk cultures of CTL against TNP-self, FITC-self, and AED-self recognized self determinants associated with the N/C1 domains of both D(d) and L(d) products. A number of allo- and hapten-specific CTL clones were also tested for recognition of the recombinant MHC products. The allo clones specific for L(d) or D(d) antigens recognized the respective N/C1-associated determinants. In addition, all clones generated against H-2(q) and known to cross-react with H-2D(d) antigens recognized determinants associated with the N/C1-associated D(d) determinants. Thus, some of the results obtained with CTL parallel, whereas others contrast with, those findings obtained with monoclonal anti-H-2 antibodies. Similar to the observations made with the monoclonal antibodies, no determinant as defined by T cells has been found to be lost as a result of the interaction between the N/C1 and C2/M domains of the L(d) and D(d) proteins. Nor did our studies detect the presence of new antigens resulting from the interaction of these gene products. However, the present T cell findings continue to contrast previous results demonstrating that antibody interaction with class I products includes recognition of C2/M-associated epitopes.
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UR - http://www.scopus.com/inward/citedby.url?scp=0021910929&partnerID=8YFLogxK
M3 - Article
C2 - 2578159
AN - SCOPUS:0021910929
VL - 134
SP - 677
EP - 683
JO - Journal of Immunology
JF - Journal of Immunology
SN - 0022-1767
IS - 2
ER -