Molecular identification of T4 and T5 genotypes in isolates from Acanthamoeba keratitis patients

D. R. Ledee, A. Iovieno, Darlene Miller, N. Mandal, Mara Diaz, J. Fell, M. E. Fini, Eduardo C Alfonso

Research output: Contribution to journalArticle

60 Citations (Scopus)

Abstract

Acanthamoeba keratitis (AK) is a rare but sight-threatening ocular infection. Outbreaks have been associated with contaminated water and contact lens wear. The epidemiology and pathology may be associated with unique genotypes. We determined the Rns genotype for 37 clinical isolates from 23 patients presenting at the University of Miami Bascom Palmer Eye Institute with confirmed AK infections in 2006 to 2008. The genus-specific ASA.S1 amplicon allowed for rapid genotyping of the nonaxenic cultures. Of the 37 isolates, 36 were of the T4 genotype. Within this group, 13 unique diagnostic fragment 3 sequences were identified, 3 of which were not in GenBank. The 37th isolate was a T5, the first in the United States and second worldwide to be found in AK. For five patients with isolates from the cornea and contact lens/case, identical sequences within each patient cluster were observed, confirming the link between contact lens contamination and AK infection. Genotyping is an important tool in the epidemiological study of AK. In this study, it allowed for the detection of new strains and provided an etiological link between source and infection. Additionally, it can allow for accurate categorizing of physiological differences, such as strain virulence, between isolates and clades.

Original languageEnglish
Pages (from-to)1458-1462
Number of pages5
JournalJournal of Clinical Microbiology
Volume47
Issue number5
DOIs
StatePublished - May 1 2009

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Acanthamoeba Keratitis
Genotype
Contact Lenses
Infection
Eye Infections
Nucleic Acid Databases
Cornea
Disease Outbreaks
Virulence
Epidemiologic Studies
Epidemiology
Pathology
Water

ASJC Scopus subject areas

  • Microbiology (medical)

Cite this

Molecular identification of T4 and T5 genotypes in isolates from Acanthamoeba keratitis patients. / Ledee, D. R.; Iovieno, A.; Miller, Darlene; Mandal, N.; Diaz, Mara; Fell, J.; Fini, M. E.; Alfonso, Eduardo C.

In: Journal of Clinical Microbiology, Vol. 47, No. 5, 01.05.2009, p. 1458-1462.

Research output: Contribution to journalArticle

Ledee, D. R. ; Iovieno, A. ; Miller, Darlene ; Mandal, N. ; Diaz, Mara ; Fell, J. ; Fini, M. E. ; Alfonso, Eduardo C. / Molecular identification of T4 and T5 genotypes in isolates from Acanthamoeba keratitis patients. In: Journal of Clinical Microbiology. 2009 ; Vol. 47, No. 5. pp. 1458-1462.
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