Molecular events in late stages of T-cell functional maturation

P. J. Nelson, R. L. Geller, E. Podack, F. H. Bach

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Peripheral blood lymphocytes activated with either the calcium ionophore A23187 or the combination of anti-CD2 monoclonal antibodies, 9.6+VIT13, undergo blast formation and proliferation but do not develop cytolytic activity. These proliferating blasts, referred to as pre-effector blasts because they do not yet express cytolytic function, respond to stimulation with interleukin-2 (IL-2) by further proliferation and development of cytolytic activity, i.e. they become effector cells. Pre-effector blasts activated with 9.6+VIT13, but not A23187-activated pre-effector blasts, also respond to stimulation with interferon-gamma (IFN-γ) by becoming cytolytic effector cells. This report examines gene expression (by Northern blot analysis) in pre-effector blasts and during the transition from the pre-effector to the effector stage. The data presented here provide further support for the concept that A23187 activation drives T cells to become dividing blasts that are appropriately referred to as 'pre-effector' cells in that these blasts do not express transcripts for granzyme A or perforin mRNA but are driven by IL-2 to do so in parallel with the acquisition of cytotoxic function. Cells are apparently driven by 9.6+VIT13 to a later stage of functional maturation than by A23187 activation; 9.6+VIT13-activated pre-effector blasts express mRNA for both granzyme A and perforin, even though these blasts do not express cytolytic activity. Activation via A23187 results in lower expression of the proto-oncogene c-myb relative to that found in either 9.6+VIT13 of OKT3-activated cells.

Original languageEnglish
Pages (from-to)311-320
Number of pages10
JournalScandinavian Journal of Immunology
Volume35
Issue number3
StatePublished - Jan 1 1992
Externally publishedYes

Fingerprint

Calcimycin
T-Lymphocytes
Granzymes
Perforin
Interleukin-2
myb Genes
Muromonab-CD3
Messenger RNA
Calcium Ionophores
Northern Blotting
Interferon-gamma
Monoclonal Antibodies
Lymphocytes
Gene Expression

ASJC Scopus subject areas

  • Immunology

Cite this

Nelson, P. J., Geller, R. L., Podack, E., & Bach, F. H. (1992). Molecular events in late stages of T-cell functional maturation. Scandinavian Journal of Immunology, 35(3), 311-320.

Molecular events in late stages of T-cell functional maturation. / Nelson, P. J.; Geller, R. L.; Podack, E.; Bach, F. H.

In: Scandinavian Journal of Immunology, Vol. 35, No. 3, 01.01.1992, p. 311-320.

Research output: Contribution to journalArticle

Nelson, PJ, Geller, RL, Podack, E & Bach, FH 1992, 'Molecular events in late stages of T-cell functional maturation', Scandinavian Journal of Immunology, vol. 35, no. 3, pp. 311-320.
Nelson PJ, Geller RL, Podack E, Bach FH. Molecular events in late stages of T-cell functional maturation. Scandinavian Journal of Immunology. 1992 Jan 1;35(3):311-320.
Nelson, P. J. ; Geller, R. L. ; Podack, E. ; Bach, F. H. / Molecular events in late stages of T-cell functional maturation. In: Scandinavian Journal of Immunology. 1992 ; Vol. 35, No. 3. pp. 311-320.
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