Molecular epidemiologic evaluation of transmissibility and virulence of Mycobacterium tuberculosis

Jeanne T. Rhee, Amy S. Piatek, Peter M. Small, Lisa M. Harris, Sandra Chaparro, Fred Russell Kramer, David Alland

Research output: Contribution to journalArticle

51 Citations (Scopus)

Abstract

Discovery of genotypic markers associated with increased transmissibility in Mycobacterium tuberculosis would represent an important step in advancing mycobacterial virulence studies. M. tuberculosis strains may be classified into one of three genotypes on the basis of the presence of specific nucleotide substitutions in codon 463 of the katG gene (katG-463) and codon 95 of the gyrA gene (gyrA-95). It has previously been reported that two of these three genotypes are associated with increased IS6110-based clustering, a potential proxy of virulence. We designed a case-control analysis of U.S.-born patients with tuberculosis in San Francisco, Calif., between 1991 and 1997 to investigate associations between katG-463 and gyrA- 95 genotypes and epidemiologically determined measures of strain-specific infectivity and pathogenicity and IS6110-based clustering status. We used a new class of molecular probes called molecular beacons to genotype the isolates rapidly. Infectivity was defined as the propensity of isolates to cause tuberculin skin test conversions among named contacts, and pathogenicity was defined as their propensity to cause active disease among named contacts. The molecular beacon assay was a simple and reproducible method for the detection of known single nucleotide polymorphisms in large numbers of clinical M. tuberculosis isolates. The results showed that no genotype of the katG-463- and gyrA-95-based classification system was associated with increased infectivity and pathogenicity or with increased IS6110-based clustering in San Francisco during the study period. We speculate that molecular epidemiologic studies investigating clinically relevant outcomes may contribute to the knowledge of the significance of laboratory-derived virulence factors in the propagation of tuberculosis in human communities.

Original languageEnglish
Pages (from-to)1764-1770
Number of pages7
JournalJournal of Clinical Microbiology
Volume37
Issue number6
StatePublished - May 29 1999
Externally publishedYes

Fingerprint

Mycobacterium tuberculosis
Virulence
Genotype
Cluster Analysis
San Francisco
Codon
Tuberculosis
Molecular Probes
Tuberculin Test
Proxy
Virulence Factors
Skin Tests
Genes
Single Nucleotide Polymorphism
Epidemiologic Studies
Nucleotides

ASJC Scopus subject areas

  • Microbiology (medical)
  • Microbiology

Cite this

Rhee, J. T., Piatek, A. S., Small, P. M., Harris, L. M., Chaparro, S., Kramer, F. R., & Alland, D. (1999). Molecular epidemiologic evaluation of transmissibility and virulence of Mycobacterium tuberculosis. Journal of Clinical Microbiology, 37(6), 1764-1770.

Molecular epidemiologic evaluation of transmissibility and virulence of Mycobacterium tuberculosis. / Rhee, Jeanne T.; Piatek, Amy S.; Small, Peter M.; Harris, Lisa M.; Chaparro, Sandra; Kramer, Fred Russell; Alland, David.

In: Journal of Clinical Microbiology, Vol. 37, No. 6, 29.05.1999, p. 1764-1770.

Research output: Contribution to journalArticle

Rhee, JT, Piatek, AS, Small, PM, Harris, LM, Chaparro, S, Kramer, FR & Alland, D 1999, 'Molecular epidemiologic evaluation of transmissibility and virulence of Mycobacterium tuberculosis', Journal of Clinical Microbiology, vol. 37, no. 6, pp. 1764-1770.
Rhee, Jeanne T. ; Piatek, Amy S. ; Small, Peter M. ; Harris, Lisa M. ; Chaparro, Sandra ; Kramer, Fred Russell ; Alland, David. / Molecular epidemiologic evaluation of transmissibility and virulence of Mycobacterium tuberculosis. In: Journal of Clinical Microbiology. 1999 ; Vol. 37, No. 6. pp. 1764-1770.
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