TY - JOUR
T1 - Molecular dissection of mouse soluble guanylyl cyclase α1 promoter
AU - Vazquez-Padron, Roberto I.
AU - Pham, Si M.
AU - Pang, Manhui
AU - Li, Sen
AU - Aïtouche, Abdelouahab
PY - 2004/1/30
Y1 - 2004/1/30
N2 - Soluble guanylyl cyclase (sGC) is the only known receptor for nitric oxide (NO) and is downregulated in aging and hypertension. Little is known about sGC gene transcriptional regulation. In order to characterize the sGC transcriptional system, we cloned and sequenced the 5′ flanking region of mouse sGC α1 gene (AY116663). Structurally, it is a non-canonical TATA-less promoter that we mapped to chromosome 3 with many putative regulation sites for Sp-1, NF-κB, and AP-1 transcription factors amongst others, and two (TG:CA)n dinucleotide microsatellites near the transcriptional start point. The cloned upstream sequence produced a 5-fold increase in luciferase activity in Cos7, HeLa, NIH3T3, and 293 cells as well as in mouse VSMC-like kidney mesangial cells. In the latter cell type, we showed that sGC α1 promoter activity was dependent on the presence of its 5′ unstranslated region (5′UTR).
AB - Soluble guanylyl cyclase (sGC) is the only known receptor for nitric oxide (NO) and is downregulated in aging and hypertension. Little is known about sGC gene transcriptional regulation. In order to characterize the sGC transcriptional system, we cloned and sequenced the 5′ flanking region of mouse sGC α1 gene (AY116663). Structurally, it is a non-canonical TATA-less promoter that we mapped to chromosome 3 with many putative regulation sites for Sp-1, NF-κB, and AP-1 transcription factors amongst others, and two (TG:CA)n dinucleotide microsatellites near the transcriptional start point. The cloned upstream sequence produced a 5-fold increase in luciferase activity in Cos7, HeLa, NIH3T3, and 293 cells as well as in mouse VSMC-like kidney mesangial cells. In the latter cell type, we showed that sGC α1 promoter activity was dependent on the presence of its 5′ unstranslated region (5′UTR).
KW - Dinucleotide microsatellites
KW - Promoter
KW - Soluble guanylyl cyclase
KW - Transcriptional regulation
KW - UTR regions
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U2 - 10.1016/j.bbrc.2003.12.078
DO - 10.1016/j.bbrc.2003.12.078
M3 - Article
C2 - 14715267
AN - SCOPUS:0347356245
VL - 314
SP - 208
EP - 214
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 1
ER -