Molecular dissection of mouse soluble guanylyl cyclase α1 promoter

Roberto I. Vazquez-Padron; Si M. Pham; Manhui Pang; Sen Li; Abdelouahab Aïtouche

doi:10.1016/j.bbrc.2003.12.078

Molecular dissection of mouse soluble guanylyl cyclase α₁ promoter

Roberto I. Vazquez-Padron, Si M. Pham, Manhui Pang, Sen Li, Abdelouahab Aïtouche

Surgery

Research output: Contribution to journal › Article › peer-review

13 Scopus citations

Abstract

Soluble guanylyl cyclase (sGC) is the only known receptor for nitric oxide (NO) and is downregulated in aging and hypertension. Little is known about sGC gene transcriptional regulation. In order to characterize the sGC transcriptional system, we cloned and sequenced the 5′ flanking region of mouse sGC α₁ gene (AY116663). Structurally, it is a non-canonical TATA-less promoter that we mapped to chromosome 3 with many putative regulation sites for Sp-1, NF-κB, and AP-1 transcription factors amongst others, and two (TG:CA)_n dinucleotide microsatellites near the transcriptional start point. The cloned upstream sequence produced a 5-fold increase in luciferase activity in Cos7, HeLa, NIH3T3, and 293 cells as well as in mouse VSMC-like kidney mesangial cells. In the latter cell type, we showed that sGC α₁ promoter activity was dependent on the presence of its 5′ unstranslated region (5′UTR).

Original language	English (US)
Pages (from-to)	208-214
Number of pages	7
Journal	Biochemical and biophysical research communications
Volume	314
Issue number	1
DOIs	https://doi.org/10.1016/j.bbrc.2003.12.078
State	Published - Jan 30 2004

Keywords

Dinucleotide microsatellites
Promoter
Soluble guanylyl cyclase
Transcriptional regulation
UTR regions

ASJC Scopus subject areas

Biochemistry
Biophysics
Molecular Biology

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title = "Molecular dissection of mouse soluble guanylyl cyclase α1 promoter",

abstract = "Soluble guanylyl cyclase (sGC) is the only known receptor for nitric oxide (NO) and is downregulated in aging and hypertension. Little is known about sGC gene transcriptional regulation. In order to characterize the sGC transcriptional system, we cloned and sequenced the 5′ flanking region of mouse sGC α1 gene (AY116663). Structurally, it is a non-canonical TATA-less promoter that we mapped to chromosome 3 with many putative regulation sites for Sp-1, NF-κB, and AP-1 transcription factors amongst others, and two (TG:CA)n dinucleotide microsatellites near the transcriptional start point. The cloned upstream sequence produced a 5-fold increase in luciferase activity in Cos7, HeLa, NIH3T3, and 293 cells as well as in mouse VSMC-like kidney mesangial cells. In the latter cell type, we showed that sGC α1 promoter activity was dependent on the presence of its 5′ unstranslated region (5′UTR).",

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AU - Vazquez-Padron, Roberto I.

AU - Pham, Si M.

AU - Pang, Manhui

AU - Li, Sen

AU - Aïtouche, Abdelouahab

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N2 - Soluble guanylyl cyclase (sGC) is the only known receptor for nitric oxide (NO) and is downregulated in aging and hypertension. Little is known about sGC gene transcriptional regulation. In order to characterize the sGC transcriptional system, we cloned and sequenced the 5′ flanking region of mouse sGC α1 gene (AY116663). Structurally, it is a non-canonical TATA-less promoter that we mapped to chromosome 3 with many putative regulation sites for Sp-1, NF-κB, and AP-1 transcription factors amongst others, and two (TG:CA)n dinucleotide microsatellites near the transcriptional start point. The cloned upstream sequence produced a 5-fold increase in luciferase activity in Cos7, HeLa, NIH3T3, and 293 cells as well as in mouse VSMC-like kidney mesangial cells. In the latter cell type, we showed that sGC α1 promoter activity was dependent on the presence of its 5′ unstranslated region (5′UTR).

AB - Soluble guanylyl cyclase (sGC) is the only known receptor for nitric oxide (NO) and is downregulated in aging and hypertension. Little is known about sGC gene transcriptional regulation. In order to characterize the sGC transcriptional system, we cloned and sequenced the 5′ flanking region of mouse sGC α1 gene (AY116663). Structurally, it is a non-canonical TATA-less promoter that we mapped to chromosome 3 with many putative regulation sites for Sp-1, NF-κB, and AP-1 transcription factors amongst others, and two (TG:CA)n dinucleotide microsatellites near the transcriptional start point. The cloned upstream sequence produced a 5-fold increase in luciferase activity in Cos7, HeLa, NIH3T3, and 293 cells as well as in mouse VSMC-like kidney mesangial cells. In the latter cell type, we showed that sGC α1 promoter activity was dependent on the presence of its 5′ unstranslated region (5′UTR).

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