Molecular cloning of the canine CD44 antigen cDNA

Kerstin F. Milde, Rodolfo Alejandro, Daniel H. Mintz, Ricardo L. Pastori

Research output: Contribution to journalArticlepeer-review

7 Scopus citations

Abstract

Molecular cloning of the dog homologue of the human CD44 was achieved using RT/PCR. A 1055 bp cDNA has a deduced amino acid sequence of 351 residues, 338 of them correspond to the mature protein. Nine conserved cysteine residues were found. The extracellular region contains a single link superfamily domain on the N-terminal part and potential post-translational modification sites as: N- and O-linked glycosylation sites and chondroitin sulfate attachment sites. Three mRNAs of 2.2, 3.8 and 4.4 kb were identified on Northern blot analysis and Western blot hybridization revealed a 85-90 kDa protein expressed in lymph node tissue.

Original languageEnglish (US)
Pages (from-to)112-114
Number of pages3
JournalBBA - Gene Structure and Expression
Volume1218
Issue number1
DOIs
StatePublished - May 17 1994

Keywords

  • CD44
  • cDNA
  • Membrane glycoprotein
  • thymus RNA

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Genetics
  • Structural Biology

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