Molecular cloning of the canine CD44 antigen cDNA

Kerstin F. Milde, Rodolfo Alejandro, Daniel H. Mintz, Ricardo Pastori

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Molecular cloning of the dog homologue of the human CD44 was achieved using RT/PCR. A 1055 bp cDNA has a deduced amino acid sequence of 351 residues, 338 of them correspond to the mature protein. Nine conserved cysteine residues were found. The extracellular region contains a single link superfamily domain on the N-terminal part and potential post-translational modification sites as: N- and O-linked glycosylation sites and chondroitin sulfate attachment sites. Three mRNAs of 2.2, 3.8 and 4.4 kb were identified on Northern blot analysis and Western blot hybridization revealed a 85-90 kDa protein expressed in lymph node tissue.

Original languageEnglish
Pages (from-to)112-114
Number of pages3
JournalBBA - Gene Structure and Expression
Volume1218
Issue number1
DOIs
StatePublished - May 17 1994

Fingerprint

CD44 Antigens
Cloning
Molecular Cloning
Canidae
Complementary DNA
Glycosylation
Chondroitin Sulfates
Post Translational Protein Processing
Northern Blotting
Cysteine
Amino Acid Sequence
Proteins
Lymph Nodes
Western Blotting
Dogs
Tissue
Amino Acids
Polymerase Chain Reaction
Messenger RNA

Keywords

  • CD44
  • cDNA
  • Membrane glycoprotein
  • thymus RNA

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Genetics
  • Structural Biology

Cite this

Molecular cloning of the canine CD44 antigen cDNA. / Milde, Kerstin F.; Alejandro, Rodolfo; Mintz, Daniel H.; Pastori, Ricardo.

In: BBA - Gene Structure and Expression, Vol. 1218, No. 1, 17.05.1994, p. 112-114.

Research output: Contribution to journalArticle

Milde, Kerstin F. ; Alejandro, Rodolfo ; Mintz, Daniel H. ; Pastori, Ricardo. / Molecular cloning of the canine CD44 antigen cDNA. In: BBA - Gene Structure and Expression. 1994 ; Vol. 1218, No. 1. pp. 112-114.
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