Molecular cloning of orangutan and gibbon MHC class I cDNA

The HLA-A and -B loci diverged over 30 million years ago

Zheng W. Chen, Stephen N. McAdam, Austin L. Hughes, Alex L. Dogon, Norman L. Letvin, David Watkins

Research output: Contribution to journalArticle

48 Citations (Scopus)

Abstract

To investigate whether the classical HLA MHC class I loci have been preserved during evolution of the primates, we have cloned, sequenced, and expressed eight MHC class I cDNA from orangutan and gibbon lymphocytes. Both the HLA-A and -B loci are present in both of these species. In fact, lymphocytes from the orangutan expressed three HLA-B-related gene products, suggesting that the ancestral homologue of the HLA-B locus had undergone a duplication in this species. Interestingly, several amino acid motifs thought to be important in the Ag-presenting function of MHC class I molecules were preserved in the Ag-recognition sites of the orangutan and gibbon MHC class I molecules. Finally, these findings suggest that the recombination event between the HLA-A and -E loci occurred over 38 million years ago. These data indicate that the HLA-A and -B loci are extremely stable and that recombination between them is rare. Furthermore, the data presented here argue against the role of concerted evolution in the evolution of primate MHC class I molecules.

Original languageEnglish
Pages (from-to)2547-2554
Number of pages8
JournalJournal of Immunology
Volume148
Issue number8
StatePublished - Apr 15 1992
Externally publishedYes

Fingerprint

Pongo
Hylobates
HLA-A Antigens
HLA-B Antigens
Molecular Cloning
Complementary DNA
Primates
Genetic Recombination
Lymphocytes
Amino Acid Motifs
Genes

ASJC Scopus subject areas

  • Immunology

Cite this

Chen, Z. W., McAdam, S. N., Hughes, A. L., Dogon, A. L., Letvin, N. L., & Watkins, D. (1992). Molecular cloning of orangutan and gibbon MHC class I cDNA: The HLA-A and -B loci diverged over 30 million years ago. Journal of Immunology, 148(8), 2547-2554.

Molecular cloning of orangutan and gibbon MHC class I cDNA : The HLA-A and -B loci diverged over 30 million years ago. / Chen, Zheng W.; McAdam, Stephen N.; Hughes, Austin L.; Dogon, Alex L.; Letvin, Norman L.; Watkins, David.

In: Journal of Immunology, Vol. 148, No. 8, 15.04.1992, p. 2547-2554.

Research output: Contribution to journalArticle

Chen, Zheng W. ; McAdam, Stephen N. ; Hughes, Austin L. ; Dogon, Alex L. ; Letvin, Norman L. ; Watkins, David. / Molecular cloning of orangutan and gibbon MHC class I cDNA : The HLA-A and -B loci diverged over 30 million years ago. In: Journal of Immunology. 1992 ; Vol. 148, No. 8. pp. 2547-2554.
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