Molecular cloning of cDNA that encode MHC class I molecules from a new world primate (Saguinus oedipus)

Natural selection acts at positions that may affect peptide presentation to T cells

David Watkins, Norman L. Letvin, Austin L. Hughes, Thomas F. Tedder

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

To investigate the evolutionary pressures that drive the generation of polymorphism in primate MHC class I molecules, three cDNA that encode MHC class I alleles from a New World monkey, the cotton-top tamarin (Saguinus oedipus), were cloned and sequenced. These tamarin MHC class I alleles contained amino acid substitutions not found in any of the previously sequenced human MHC class I alleles. Moreover, the majority of these unique amino acid substitutions was located in the Ag recognition site at positions that have been shown to be critical in the presentation of viral peptides to T cells in mice and humans. These data suggest that selective pressures on MHC class I molecules preferentially act on the Ag recognition site and that the peptide binding or presenting functions of these molecules may drive the generation of MHC class I polymorphism. The novel Ag recognition sites of the tamarin MHC class I molecules, in addition to their restricted polymorphism, might account for the unusual susceptibility of the cotton-top tamarin to human pathogens.

Original languageEnglish
Pages (from-to)1136-1143
Number of pages8
JournalJournal of Immunology
Volume144
Issue number3
StatePublished - Feb 1 1990
Externally publishedYes

Fingerprint

Saguinus
Genetic Selection
Molecular Cloning
Primates
Complementary DNA
Alleles
Amino Acid Substitution
T-Lymphocytes
Peptides
Platyrrhini
Peptide T
Binding Sites
Pressure

ASJC Scopus subject areas

  • Immunology

Cite this

Molecular cloning of cDNA that encode MHC class I molecules from a new world primate (Saguinus oedipus) : Natural selection acts at positions that may affect peptide presentation to T cells. / Watkins, David; Letvin, Norman L.; Hughes, Austin L.; Tedder, Thomas F.

In: Journal of Immunology, Vol. 144, No. 3, 01.02.1990, p. 1136-1143.

Research output: Contribution to journalArticle

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