Molecular cloning of a brain-specific calcium/calmodulin-dependent protein kinase.

C. R. Lin, M. S. Kapiloff, S. Durgerian, K. Tatemoto, A. F. Russo, P. Hanson, H. Schulman, M. G. Rosenfeld

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217 Scopus citations


A calcium/calmodulin-dependent protein kinase type II (CaM-K) alpha-subunit cDNA has been cloned from rat brain. This enzyme is encoded by a 5.1-kilobase mRNA expressed exclusively in the brain. Hybridization histochemistry reveals that the CaM-K mRNA expression corresponds to the distribution of the immunoreactive alpha-subunit protein, suggesting that the high enzyme levels in specific brain areas reflect regional differences in gene expression. The sequence of CaM-K alpha-subunit cDNA indicates a 478-amino acid (54-kDa) protein with three functional domains. The domain organization suggests a structural model for calcium/calmodulin-dependent and independent states that might subserve short- and long-term responses to transient stimuli.

Original languageEnglish (US)
Pages (from-to)5962-5966
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Issue number16
StatePublished - Aug 1987
Externally publishedYes

ASJC Scopus subject areas

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