Molecular cloning of a brain-specific calcium/calmodulin-dependent protein kinase.

C. R. Lin, Michael S Kapiloff, S. Durgerian, K. Tatemoto, A. F. Russo, P. Hanson, H. Schulman, M. G. Rosenfeld

Research output: Contribution to journalArticle

213 Citations (Scopus)

Abstract

A calcium/calmodulin-dependent protein kinase type II (CaM-K) alpha-subunit cDNA has been cloned from rat brain. This enzyme is encoded by a 5.1-kilobase mRNA expressed exclusively in the brain. Hybridization histochemistry reveals that the CaM-K mRNA expression corresponds to the distribution of the immunoreactive alpha-subunit protein, suggesting that the high enzyme levels in specific brain areas reflect regional differences in gene expression. The sequence of CaM-K alpha-subunit cDNA indicates a 478-amino acid (54-kDa) protein with three functional domains. The domain organization suggests a structural model for calcium/calmodulin-dependent and independent states that might subserve short- and long-term responses to transient stimuli.

Original languageEnglish
Pages (from-to)5962-5966
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume84
Issue number16
StatePublished - Aug 1 1987
Externally publishedYes

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Calcium-Calmodulin-Dependent Protein Kinases
Molecular Cloning
Calcium-Calmodulin-Dependent Protein Kinase Type 2
Brain
Complementary DNA
Messenger RNA
Structural Models
Protein Subunits
Enzymes
Calmodulin
Calcium
Gene Expression
Amino Acids
Proteins

ASJC Scopus subject areas

  • General
  • Genetics

Cite this

Lin, C. R., Kapiloff, M. S., Durgerian, S., Tatemoto, K., Russo, A. F., Hanson, P., ... Rosenfeld, M. G. (1987). Molecular cloning of a brain-specific calcium/calmodulin-dependent protein kinase. Proceedings of the National Academy of Sciences of the United States of America, 84(16), 5962-5966.

Molecular cloning of a brain-specific calcium/calmodulin-dependent protein kinase. / Lin, C. R.; Kapiloff, Michael S; Durgerian, S.; Tatemoto, K.; Russo, A. F.; Hanson, P.; Schulman, H.; Rosenfeld, M. G.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 84, No. 16, 01.08.1987, p. 5962-5966.

Research output: Contribution to journalArticle

Lin, CR, Kapiloff, MS, Durgerian, S, Tatemoto, K, Russo, AF, Hanson, P, Schulman, H & Rosenfeld, MG 1987, 'Molecular cloning of a brain-specific calcium/calmodulin-dependent protein kinase.', Proceedings of the National Academy of Sciences of the United States of America, vol. 84, no. 16, pp. 5962-5966.
Lin, C. R. ; Kapiloff, Michael S ; Durgerian, S. ; Tatemoto, K. ; Russo, A. F. ; Hanson, P. ; Schulman, H. ; Rosenfeld, M. G. / Molecular cloning of a brain-specific calcium/calmodulin-dependent protein kinase. In: Proceedings of the National Academy of Sciences of the United States of America. 1987 ; Vol. 84, No. 16. pp. 5962-5966.
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