Molecular cloning of a brain-specific calcium/calmodulin-dependent protein kinase.

C. R. Lin; M. S. Kapiloff; S. Durgerian; K. Tatemoto; A. F. Russo; P. Hanson; H. Schulman; M. G. Rosenfeld

doi:10.1073/pnas.84.16.5962

Molecular cloning of a brain-specific calcium/calmodulin-dependent protein kinase.

C. R. Lin, M. S. Kapiloff, S. Durgerian, K. Tatemoto, A. F. Russo, P. Hanson, H. Schulman, M. G. Rosenfeld

Research output: Contribution to journal › Article › peer-review

217 Scopus citations

Abstract

A calcium/calmodulin-dependent protein kinase type II (CaM-K) alpha-subunit cDNA has been cloned from rat brain. This enzyme is encoded by a 5.1-kilobase mRNA expressed exclusively in the brain. Hybridization histochemistry reveals that the CaM-K mRNA expression corresponds to the distribution of the immunoreactive alpha-subunit protein, suggesting that the high enzyme levels in specific brain areas reflect regional differences in gene expression. The sequence of CaM-K alpha-subunit cDNA indicates a 478-amino acid (54-kDa) protein with three functional domains. The domain organization suggests a structural model for calcium/calmodulin-dependent and independent states that might subserve short- and long-term responses to transient stimuli.

Original language	English (US)
Pages (from-to)	5962-5966
Number of pages	5
Journal	Proceedings of the National Academy of Sciences of the United States of America
Volume	84
Issue number	16
DOIs	https://doi.org/10.1073/pnas.84.16.5962
State	Published - Aug 1987
Externally published	Yes

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Molecular cloning of a brain-specific calcium/calmodulin-dependent protein kinase. / Lin, C. R.; Kapiloff, M. S.; Durgerian, S.; Tatemoto, K.; Russo, A. F.; Hanson, P.; Schulman, H.; Rosenfeld, M. G.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 84, No. 16, 08.1987, p. 5962-5966.

Research output: Contribution to journal › Article › peer-review

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abstract = "A calcium/calmodulin-dependent protein kinase type II (CaM-K) alpha-subunit cDNA has been cloned from rat brain. This enzyme is encoded by a 5.1-kilobase mRNA expressed exclusively in the brain. Hybridization histochemistry reveals that the CaM-K mRNA expression corresponds to the distribution of the immunoreactive alpha-subunit protein, suggesting that the high enzyme levels in specific brain areas reflect regional differences in gene expression. The sequence of CaM-K alpha-subunit cDNA indicates a 478-amino acid (54-kDa) protein with three functional domains. The domain organization suggests a structural model for calcium/calmodulin-dependent and independent states that might subserve short- and long-term responses to transient stimuli.",

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Molecular cloning of a brain-specific calcium/calmodulin-dependent protein kinase.

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