Abstract
A calcium/calmodulin-dependent protein kinase type II (CaM-K) alpha-subunit cDNA has been cloned from rat brain. This enzyme is encoded by a 5.1-kilobase mRNA expressed exclusively in the brain. Hybridization histochemistry reveals that the CaM-K mRNA expression corresponds to the distribution of the immunoreactive alpha-subunit protein, suggesting that the high enzyme levels in specific brain areas reflect regional differences in gene expression. The sequence of CaM-K alpha-subunit cDNA indicates a 478-amino acid (54-kDa) protein with three functional domains. The domain organization suggests a structural model for calcium/calmodulin-dependent and independent states that might subserve short- and long-term responses to transient stimuli.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 5962-5966 |
| Number of pages | 5 |
| Journal | Proceedings of the National Academy of Sciences of the United States of America |
| Volume | 84 |
| Issue number | 16 |
| DOIs | |
| State | Published - Aug 1987 |
ASJC Scopus subject areas
- General
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Lin, C. R., Kapiloff, M. S., Durgerian, S., Tatemoto, K., Russo, A. F., Hanson, P., Schulman, H., & Rosenfeld, M. G. (1987). Molecular cloning of a brain-specific calcium/calmodulin-dependent protein kinase. Proceedings of the National Academy of Sciences of the United States of America, 84(16), 5962-5966. https://doi.org/10.1073/pnas.84.16.5962