Abstract
A calcium/calmodulin-dependent protein kinase type II (CaM-K) alpha-subunit cDNA has been cloned from rat brain. This enzyme is encoded by a 5.1-kilobase mRNA expressed exclusively in the brain. Hybridization histochemistry reveals that the CaM-K mRNA expression corresponds to the distribution of the immunoreactive alpha-subunit protein, suggesting that the high enzyme levels in specific brain areas reflect regional differences in gene expression. The sequence of CaM-K alpha-subunit cDNA indicates a 478-amino acid (54-kDa) protein with three functional domains. The domain organization suggests a structural model for calcium/calmodulin-dependent and independent states that might subserve short- and long-term responses to transient stimuli.
| Original language | English |
|---|---|
| Pages (from-to) | 5962-5966 |
| Number of pages | 5 |
| Journal | Proceedings of the National Academy of Sciences of the United States of America |
| Volume | 84 |
| Issue number | 16 |
| State | Published - Aug 1 1987 |
| Externally published | Yes |
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ASJC Scopus subject areas
- General
- Genetics
Cite this
Molecular cloning of a brain-specific calcium/calmodulin-dependent protein kinase. / Lin, C. R.; Kapiloff, Michael S; Durgerian, S.; Tatemoto, K.; Russo, A. F.; Hanson, P.; Schulman, H.; Rosenfeld, M. G.
In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 84, No. 16, 01.08.1987, p. 5962-5966.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Molecular cloning of a brain-specific calcium/calmodulin-dependent protein kinase.
AU - Lin, C. R.
AU - Kapiloff, Michael S
AU - Durgerian, S.
AU - Tatemoto, K.
AU - Russo, A. F.
AU - Hanson, P.
AU - Schulman, H.
AU - Rosenfeld, M. G.
PY - 1987/8/1
Y1 - 1987/8/1
N2 - A calcium/calmodulin-dependent protein kinase type II (CaM-K) alpha-subunit cDNA has been cloned from rat brain. This enzyme is encoded by a 5.1-kilobase mRNA expressed exclusively in the brain. Hybridization histochemistry reveals that the CaM-K mRNA expression corresponds to the distribution of the immunoreactive alpha-subunit protein, suggesting that the high enzyme levels in specific brain areas reflect regional differences in gene expression. The sequence of CaM-K alpha-subunit cDNA indicates a 478-amino acid (54-kDa) protein with three functional domains. The domain organization suggests a structural model for calcium/calmodulin-dependent and independent states that might subserve short- and long-term responses to transient stimuli.
AB - A calcium/calmodulin-dependent protein kinase type II (CaM-K) alpha-subunit cDNA has been cloned from rat brain. This enzyme is encoded by a 5.1-kilobase mRNA expressed exclusively in the brain. Hybridization histochemistry reveals that the CaM-K mRNA expression corresponds to the distribution of the immunoreactive alpha-subunit protein, suggesting that the high enzyme levels in specific brain areas reflect regional differences in gene expression. The sequence of CaM-K alpha-subunit cDNA indicates a 478-amino acid (54-kDa) protein with three functional domains. The domain organization suggests a structural model for calcium/calmodulin-dependent and independent states that might subserve short- and long-term responses to transient stimuli.
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UR - http://www.scopus.com/inward/citedby.url?scp=0023390675&partnerID=8YFLogxK
M3 - Article
C2 - 3475713
AN - SCOPUS:0023390675
VL - 84
SP - 5962
EP - 5966
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
SN - 0027-8424
IS - 16
ER -